Skip to main content
Log in

Isolation and characterization of thermophilic benzothiophene-degrading Mycobacterium sp.

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A bacterial strain, SWU-4, capable of using benzothiophene (BT) as a sole carbon and energy source was isolated from a petroleum-contaminated site in Thailand and identified by 16S rRNA gene sequence analysis to be in the genus of Mycobacterium. The strain was Gram-positive, nonspore former, and grew at 50° C. Colonies of the strain on nutrient agar were rod-shaped, smooth with a convex surface, slightly mucoid, and yellow pigmented. The thermophilic Mycobacterium sp. strain SWU-4 rapidly degraded 2% (w/v) BT at 50°C. Interestingly, this strain was able to degrade a wide variety of organosulfur compounds including thiophene, bromo(α)thiophene, and 3-methylthiophene in liquid minimum medium at 50°C, which will be beneficial for industrial applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Fedorak, P. M. (1990), in Microbial Metabolism of Organosulfur Compounds in Petroleum, Orr, W. L. and White, C. M., eds., ACS Books, Washington, DC, pp. 93–112.

    Google Scholar 

  2. Atlas, R. M. (1981), Microbiol. Rev. 45, 180–209.

    CAS  Google Scholar 

  3. Zhou, E. and Creawford, R. L. (1995), Biodegradation 6, 127–140.

    Article  CAS  Google Scholar 

  4. Allard, A. S. and Nelson, A. (1997), Int. Biodeterior. Biodegr. 39, 253–285.

    Article  CAS  Google Scholar 

  5. Fedorak, P. M. and Grbic-Galic, D. (1991), Appl. Environ. Microbiol. 57, 932–940.

    CAS  Google Scholar 

  6. Sagardia, F., Rigau, J. J., Martinez-Lahoz, A., Fuenter, F., Lopez, C., and Flores, W. (1975), Appl. Microbiol. 29, 722–725.

    CAS  Google Scholar 

  7. Finnerty, W. R., Shockley, K., and Attaway, H. (1983), Microbial Enhanced Oil Recovery, PennWell, Tulsa, OK.

    Google Scholar 

  8. Bohonos, N., Chou, T. W., and Spanggord, R. J. (1977), Jpn. J. Antibiot. 33(suppl. 8), 275–285.

    Google Scholar 

  9. Grifoll, M., Casellas, M., Bayona, J. M., and Solanas, A. M. (1992), Appl. Environ. Microbiol. 58, 2910–2917.

    CAS  Google Scholar 

  10. van Elsas, J. D. and Smalla, K. (1977), Manual of Environmental Microbiology, American Society for Microbiology, Washington, DC.

    Google Scholar 

  11. Kiyohara, H., Nagao, K., and Yana, K. (1982), Appl. Environ. Microbiol. 43, 454–457.

    Google Scholar 

  12. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R., and Phillips, G. B. (1981), Manual of Methods for General Bacteriology, American Society of Microbiology, Washington, DC.

    Google Scholar 

  13. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  14. Lane, D. J. (1991), in Nucleic Acid Techniques in Bacterial Systematics, Strackebrandt, E. and Goodfellow, M., eds., Wiley & Sons, Chichester, UK, pp. 115–175.

    Google Scholar 

  15. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994), Nucleic Acids Res. 22, 4673–4680.

    Article  CAS  Google Scholar 

  16. Kimura, M. (1980), J. Mol. Evol. 16, 111–120.

    Article  CAS  Google Scholar 

  17. Saitou, N. and Nei, M. (1987), Mol. Biol. Evol. 4, 406–425.

    CAS  Google Scholar 

  18. Felsenstein, J. (1993), Phylip (Phylogenetic Inference Package) version 3.56c, Department of Genetics, University of Washington, Seattle, WA.

    Google Scholar 

  19. Kayser, K. J., Bielaga, B. B., Jackowski, K., Oduson, O., and Kilbane, J. J. (1993), J. Gen. Microbiol. 139, 3123–3129.

    CAS  Google Scholar 

  20. Izumi, Y., Ohshiro, T., Ogino, H., Hine, Y., and Shimao, M. (1994), Appl. Environ. Microbiol. 60, 223–226.

    CAS  Google Scholar 

  21. Barksdale, L. and Kim, K. (1977), Bacteriol. Rev. 41, 217–327.

    CAS  Google Scholar 

  22. Boldrin, B., Teihm, A. and Fritzsche, C. (1993), Appl. Environ. Microbiol. 59, 1927–1930.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yuwadee Watanapokasin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Watanapokasin, Y., Nuchfoang, S., Nilwarangkoon, S. et al. Isolation and characterization of thermophilic benzothiophene-degrading Mycobacterium sp.. Appl Biochem Biotechnol 98, 301–309 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:301

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:98-100:1-9:301

Index Entries

Navigation