Abstract
A batch culture of Saccharomyces cerevisiae for the production of hexokinase was carried out in a 5-L fermentor containing 3 L of culture medium, which was in oculated with cell suspension (about 0.7 g/L), and left ferm entingat 35°C and pH 4.0. The aeration and agitation were adjusted to attain k La values of 15, 60, 135, and 230 h−1. The highest hexokinase productivity (754.6 U/[L h]) and substrate-cell conversion yield (0.21 g/g) occurred for a k La of 60 h−1. Moreover, the formation of hexokinase and cell growth are coupled events, which is in accordance with the constitutive character of this enzyme. Hexokinase formation for k La>60 h−1 was not enhanced probably owing to saturation of the respiratory pathway by oxygen.
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Silva, D.P., Pessoa, A., Roberto, IC. et al. Effect of agitation and aeration on production of hexokinase by Saccharomyces cerevisiae . Appl Biochem Biotechnol 91, 605–613 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:605
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DOI: https://doi.org/10.1385/ABAB:91-93:1-9:605