Skip to main content
Log in

Utilization of cyanobacteria in photobioreactors for orthophosphate removal from water

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The effectiveness of photosynthetic free-living and polyurethane foam (PU) immobilized Anabaena variabilis cells for, removal of orthophosphate (P) from water in batch cultures and in a photobioreactor was studied. Immobilization in PU foams was found to have a positive effect on P uptake by cyanobacteria in batch cultures. The efficiency of P uptake by immobilized cells was higher than by free-living cells. A laboratory scale photobioreactor was constructed for removal of P from water by the immobilized cyanobacteria. The photobioreactor was designed so that the growth medium (water) from a reservoir was pumped through a photobioreactor column with immobilized cyanobacteria and back to the reservoir. This created a closed system in which it was possible to measure P uptake. No leakage of cells into the photobioreactor medium reservoir was observed during the operation. The immobilized cells incorporated into a photobioreactor column removed P continuously for about 15 d. No measurable uptake was demonstrated after this period. Orthophosphate uptake efficiency of 88–92% was achieved by the photobioreactor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Sirenko, L. A., and Gavrilenko, M. Y. (1978), Water Blooms and Eutrophication, Naukova Dumka, Kiev, Russia.

    Google Scholar 

  2. Hallegraeff, G. M. (1993), Phycologia 32, 79–99.

    Google Scholar 

  3. Environmental Protection Agency (1991), National primary drinking water regulations; final rule. 40CFR Parts 141, 142, and 143. Federal Register 56, 20, 3526–97.

    Google Scholar 

  4. Garbisu, C., Hall, D. O., and Serra, L. (1993), J. Chem. Tech. Biotechnol. 57, 181–189.

    CAS  Google Scholar 

  5. Garbisu, C., Gil, J. M., Bazin, M. J., Hall, D. O., and Serra, J. L. (1991), J. Appl. Psychol. 3, 221–234.

    CAS  Google Scholar 

  6. Hall, D. O., Markov, S. A., Watanabe, Y., and Rao, K. K. (1995), Photosynthesis Res. 46, 159–167.

    Article  CAS  Google Scholar 

  7. Hall, D. O. and Rao, K. K. (1989), Chimicaoggi. 7, 40–47.

    Google Scholar 

  8. Markov, S. A. (1998), in 20th Symposium on Biotechnology for Fuels and Chemicals, Program and Abstracts, Gatlinburg, TN, ORNL, Oak Ridge, TN.

    Google Scholar 

  9. Markov, S. A., Lichtl, R. R., Rao, K. K., and Hall, D. O. (1993), Int. J. Hydrogen Energy 18, 901–906.

    Article  CAS  Google Scholar 

  10. Allen, M. and Arnon, D. I. (1955), Plant Physiol. 30, 366–372.

    Article  CAS  Google Scholar 

  11. Spiller, H. and Gunasekaran, M. (1991), Appl. Microbiol. Biotechnol. 35, 798–804.

    Article  CAS  Google Scholar 

  12. (1985), Standard Methods for Examination of Water and Wasterwater, American Public Health Association.

  13. de la Noue, J. and Proulx, D. (1988), in Algal Biotechnology, Stadler, T., Mollion, J., Verdus, M. C., Karamanos, W., Morvan, H., and Christiaen, D., eds., Elsevier Applied Science, Barking, Essex, England, UK, 159–168.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Gunasekaran.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gaffney, A.M., Markov, S.A. & Gunasekaran, M. Utilization of cyanobacteria in photobioreactors for orthophosphate removal from water. Appl Biochem Biotechnol 91, 185–193 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:185

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:91-93:1-9:185

Index Entries

Navigation