Abstract
The effectiveness of photosynthetic free-living and polyurethane foam (PU) immobilized Anabaena variabilis cells for, removal of orthophosphate (P) from water in batch cultures and in a photobioreactor was studied. Immobilization in PU foams was found to have a positive effect on P uptake by cyanobacteria in batch cultures. The efficiency of P uptake by immobilized cells was higher than by free-living cells. A laboratory scale photobioreactor was constructed for removal of P from water by the immobilized cyanobacteria. The photobioreactor was designed so that the growth medium (water) from a reservoir was pumped through a photobioreactor column with immobilized cyanobacteria and back to the reservoir. This created a closed system in which it was possible to measure P uptake. No leakage of cells into the photobioreactor medium reservoir was observed during the operation. The immobilized cells incorporated into a photobioreactor column removed P continuously for about 15 d. No measurable uptake was demonstrated after this period. Orthophosphate uptake efficiency of 88–92% was achieved by the photobioreactor.
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Sirenko, L. A., and Gavrilenko, M. Y. (1978), Water Blooms and Eutrophication, Naukova Dumka, Kiev, Russia.
Hallegraeff, G. M. (1993), Phycologia 32, 79–99.
Environmental Protection Agency (1991), National primary drinking water regulations; final rule. 40CFR Parts 141, 142, and 143. Federal Register 56, 20, 3526–97.
Garbisu, C., Hall, D. O., and Serra, L. (1993), J. Chem. Tech. Biotechnol. 57, 181–189.
Garbisu, C., Gil, J. M., Bazin, M. J., Hall, D. O., and Serra, J. L. (1991), J. Appl. Psychol. 3, 221–234.
Hall, D. O., Markov, S. A., Watanabe, Y., and Rao, K. K. (1995), Photosynthesis Res. 46, 159–167.
Hall, D. O. and Rao, K. K. (1989), Chimicaoggi. 7, 40–47.
Markov, S. A. (1998), in 20th Symposium on Biotechnology for Fuels and Chemicals, Program and Abstracts, Gatlinburg, TN, ORNL, Oak Ridge, TN.
Markov, S. A., Lichtl, R. R., Rao, K. K., and Hall, D. O. (1993), Int. J. Hydrogen Energy 18, 901–906.
Allen, M. and Arnon, D. I. (1955), Plant Physiol. 30, 366–372.
Spiller, H. and Gunasekaran, M. (1991), Appl. Microbiol. Biotechnol. 35, 798–804.
(1985), Standard Methods for Examination of Water and Wasterwater, American Public Health Association.
de la Noue, J. and Proulx, D. (1988), in Algal Biotechnology, Stadler, T., Mollion, J., Verdus, M. C., Karamanos, W., Morvan, H., and Christiaen, D., eds., Elsevier Applied Science, Barking, Essex, England, UK, 159–168.
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Gaffney, A.M., Markov, S.A. & Gunasekaran, M. Utilization of cyanobacteria in photobioreactors for orthophosphate removal from water. Appl Biochem Biotechnol 91, 185–193 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:185
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DOI: https://doi.org/10.1385/ABAB:91-93:1-9:185