Abstract
Because Nitrosomonas europaea contains ammonia-oxidizing enzyme, nitrite reductase, and nitrous oxide reductase, the conversion of ammonia to dinitrogen was tried with different reaction conditions. In aerobic reaction conditions, ammonium was converted to nitrite (NO −2 ), while under oxygen-limiting or oxygen-free conditions, NO −2 -N formed from ammonia oxidation by N. europaea was reduced to N2O and dinitrogen with 22% conversion. During denitrification, optimal pH for the production of N2O and dinitrogen was found to be 7.0–8.0. Dinitrogen was not produced in acidic pH<7.0. A low partial oxygen pressure as well as oxygen-free conditions are favorable for high production of dinitrogen.
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References
Robertson, L. A. and Kuenen, J. G. (1990), Ant. Van. Leeuwenhoek 56, 289–299.
Muller, E. B., Stouthamer, A. H., and Verseveld, H. W. (1995), Biodegradation 6, 339–349.
Bock, E., Schmidt, I., Stuven, R., and Zart, D. (1995), Arch. Microbiol. 163, 16–20.
Schmidt, I. and Bock, E. (1997), Arch. Microbiol. 167, 106–111.
Wood, P. M. (1986), in Nitrification, Prosser, J. I., ed., IRL, Oxford, pp. 39–62.
Goreau, T. J., Kaplan, W. A., Wofsy, S. C., McElroy, M. B., Valois, F. B., and Watson, S. W. (1980), Appl. Environ. Microbiol. 40, 526–532.
Miller, D. J. and Nicholas, D. J. D. (1985), J. Gen. Microbiol. 131, 2581–2584.
Hooper, A. B. (1968), Biochim. Biophys. Acta 162, 49–65.
Poth, M. (1986), Appl. Environ. Microbiol. 52, 957–959.
Zart, D., Schmidt, I., and Bock, E. (1996), in Okologie der Abwasserorganismen, Lemmer, H., Griebe, T., and Flemming, H. C., eds., Springer, Berlin, pp. 183–192.
Miller, D. J. and Wood, P. M. (1983), J. Gen. Microbiol. 129, 1645–1650.
Klapwijk, A. (1995), in Denitrification in Nitrogen Cycles, vol. 9, Golterman, H. L., ed., NATO conference series 1, Plenum, New York, pp. 241–255.
Her, J. J. and Huang, J. S. (1995), Biores. Technol. 54, 45–51.
Japanese Standards Association (1994), Japan Industrial Standards Handbook, Kankyo Sokutei, JIS, Japan.
Stuven, R., Vollmer, M., and Bock, E. (1992), Arch. Microbiol. 158, 439–443.
Prosser, J. I. (1989), Adv. Microb. Physiol. 30, 125–177.
Suzuki, I., Dular, U., and Kwok, S. C. (1974), J. Bacteriol. 120, 556–558.
Poth, M. and Focht, D. D. (1985), Appl. Environ. Microbiol. 49, 1134–1141.
Bonner, F. T. and Stedman, G. (1996), in Methods in Nitric Oxide Research, Feelisch, M. and Stamler, J. S., eds., John Wiley & Sons, New York, pp. 3–18.
Cui, X., Joannou, C. L., Hughes, M. N., and Cammack, R. (1992), FEMS Microbiol. Lett. 98, 67–70.
Ritchie, G. A. F. and Nicholas, D. J. D. (1972), Biochem. J. 126, 1181–1191.
Hopper, A. B. and Terry, K. R. (1979), Biochim. Biophys. Acta 571, 12–20.
Betlach, M. R. and Tiedje, J. M. (1981), Appl. Environ. Microbiol. 42, 1074–1084.
Zumft, W. G., Viebrock, A., and Korner, H. (1988), in The Nitrogen and Sulphur Cycles, Cole, J. A. and Ferguson, S., eds., Cambridge University Press, Cambridge, England, pp. 245–279.
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Shrestha, N.K., Hadano, S., Kamachi, T. et al. Conversion of ammonia to dinitrogen in wastewater by Nitrosomonas europaea . Appl Biochem Biotechnol 90, 221–232 (2001). https://doi.org/10.1385/ABAB:90:3:221
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DOI: https://doi.org/10.1385/ABAB:90:3:221