Abstract
Human secreted alkaline phosphatase (SEAP) was produced in a stablytransformed Spodoptera frugiperda Sf-9 insect cell line (Sfb4GalT) following infection with a recombinant Autographa californica multiple nuclear polyhedrovirus containing the SEAP gene under control of the polyhedrin promoter. An affinity chromatographic column prepared by linking 4-aminobenzylphosphonic acid to histidyl-expoxy-Sepharose was used to isolate SEAP from the cell supernatant following removal of cells and virus and 10-fold concentration through ultrafiltration. We found that the binding of SEAP on the affinity matrix follows the Langmuir isotherm model. In addition, either recycling SEAP sample through the column for 24 h or loading high SEAP concentrations resulted in a high-purity product. Some nonspecific binding of protein on the matrix occurred when low concentrations of SEAP sample were loaded. Finally, we found that SEAP binding occurs rapidly, i.e., within 30 min of adding the SEAP sample to the affinity matrix.
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Luckow, V. A. (1995), in Baculovirus Expression Systems and Biopesticides, Shuler, M. L., Wood, H. A., Granados, R. R., and Hammer, H. A., eds., Wiley-Liss, New York, pp. 51–90.
Marz, L., Altmann, F., Staudacher, E., and Kubelka, V. (1995), in Glycoprotein, vol. 29a, Montreuil, J., Vliegenthart, J. F. G., and Schachter, H., eds., Elsevier, Amsterdam, pp. 543–563.
Jarvis, D. L. (1997), in The Baculoviruses, Miller, L. K., ed., Plenum, New York, pp. 389–431.
Butters, T. D., Hughes, P. C., and Vischer, P. (1981), Biochem. Biophys. Acta 640, 672–686.
Jarvis, D. L., Kawar, Z. S., and Hollister, J. R. (1998), Curr. Opin. Biotechnol. 9, 528–533.
Hollister, J. R., Shaper, J. H., and Jarvis, D. L. (1998), Glycobiology 8, 473–480.
Millan, J. L. (1986), J. Biol. Chem. 261, 3112–3115.
Endo, T., Ohbayashi, H., Hayashi, Y., Ikehara, Y., Kochibe, N., and Kobata, A. (1988), J. Biochem. 103, 182–187.
Sofer, G. K. and Nystron, L. E. (1989), Process Chromatography, A Practical Guide, Academic, London.
Kulakosky, P. C., Shuler, M. L., and Wood, H. A. (1998), In Vitro Cell. Dev. Biol.-Anim. 34, 101–108.
Landt, M., Boltz, S. C., and Butler, L. G. (1978), Biochemistry 17, 915–919.
Davis, T. R., Trotter, K. M., Granados, R. R., and Wood, H. A. (1992), Bio/Technology 10, 1148–1150.
Vijayalakshmi, M. A. (1996), Mol. Biotechnol. 6, 347–357.
Matsumoto, I., Mizuno, Y., and Seno, N. (1979), J. Biochem. 85, 1091–1098.
Saarinen, M. A., Troutner, K. A., Gladden, S. G., Mitchell-Logean, C. M., and Murhammer, D. W. (1999), Biotechnol. Bioeng. 63, 612–617.
Heukesshoven, J. and Denick, R. (1985), Electrophoresis 6, 103–112.
Ayres, M. D. Howard, S. C., Kuzio, J., Lopez-Ferber, M., and Possee, R. D. (1994), Virology 202, 586–605.
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Zhang, F., Wolff, M.W., Williams, D. et al. Affinity purification of secreted alkaline phosphatase produced by baculovirus expression vector system. Appl Biochem Biotechnol 90, 125–136 (2001). https://doi.org/10.1385/ABAB:90:2:125
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DOI: https://doi.org/10.1385/ABAB:90:2:125