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Xylitol production from wood hydrolyzates by entrapped Debaryomyces hansenii and Candida guilliermondii cells

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Abstract

Debaryomyces hansenii cells were entrapped in Ca-alginate beads and used for producing xylitol from wood hydrolyzates. Batch experiments showed that bioconversion was severely hindered when Ca-alginate beads were hardened with Al3+ solutions. As an alternative to Al3+ hardening, the improvements in both mechanical stability of bioparticles and fermenting ability of the immobilized system derived from using increased concentrations of sodium alginate were assessed. The best results were obtained using a 4% (w/v) Na-alginate solution in the gelification step. This concentration was selected to perform continuous fermentations in a packed-bed reactor using raw or charcoal-treated hydrolyzates (15.5 g of xylose/L) with two different yeasts: Candida guilliermondii and Debaryomyces hansenii. With a final cell concentration of about 50 g of cells/L (0.075 g of cells/g of beads), the volumetric productivities reached with these yeasts in media made from charcoal-treated hydrolyzates were 0.58 and 0.91 g/L·h, respectively.

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Authors and Affiliations

  1. Chemical Engineering Department, University of Vigo (Campus Ourense), 32004, Ourense, Spain

    Jose Manuel Domínguez, Jose Manuel Cruz, Herminia Domínguez & Juan Carlos Parajó

  2. Chemical Engineering Department, Instituto de Investigaciones Tecnológicas, University of Santiago de Compostela (Campus Lugo), 27002, Lugo, Spain

    Enrique Roca

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  1. Jose Manuel Domínguez
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  2. Jose Manuel Cruz
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  3. Enrique Roca
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  4. Herminia Domínguez
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  5. Juan Carlos Parajó
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Correspondence to Jose Manuel Domínguez.

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Domínguez, J.M., Cruz, J.M., Roca, E. et al. Xylitol production from wood hydrolyzates by entrapped Debaryomyces hansenii and Candida guilliermondii cells. Appl Biochem Biotechnol 81, 119–130 (1999). https://doi.org/10.1385/ABAB:81:2:119

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  • DOI: https://doi.org/10.1385/ABAB:81:2:119

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