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Lignin peroxidase production by Streptomyces viridosporus T7A

Nitrogen nutrition optimization using glucose as carbon source

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Abstract

The production of lignin peroxidase by Streptomyces viridosporus T7A was studied in submerged batch fermentations using growth media containing 6.5 g/L yeast extract and 2.5–10.0 g/L glucose, corresponding to carbon to nitrogen (C/N) ratios from 7.1–12.4. The kinetics for biomass and enzyme accumulation and glucose consumption were followed allowing definition of optimized conditions for enzyme production. Considering the physiological response of the microorganism in relation to enzyme production, a sharp increase on enzyme activity was consistently observed upon glucose depletion, indicating glucose regulation. In accordance to that the plot of maximal enzyme vs maximal enzyme per gram of glucose consumption showed a linear inversely proportional relationship, indicating that the characteristics of the metabolic pool at the studied C/N ratios affected enzyme biosynthesis even after glucose depletion.

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Authors and Affiliations

  1. Programa de Engenharia Química, COPPE, 2 1949-900, Rio de Janeiro, RJ, Brazil

    Edna M. M. Oliveira & Elba P. S. Bon

  2. Departamento de Bioquímica, Instituto de Química, UFRJ, 2 1949-900, Rio de Janeiro, RJ, Brazil

    José E. Zerbini

Authors
  1. José E. Zerbini
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  2. Edna M. M. Oliveira
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  3. Elba P. S. Bon
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Correspondence to Elba P. S. Bon.

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Zerbini, J.E., Oliveira, E.M.M. & Bon, E.P.S. Lignin peroxidase production by Streptomyces viridosporus T7A. Appl Biochem Biotechnol 79, 681–688 (1999). https://doi.org/10.1385/ABAB:79:1-3:681

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  • DOI: https://doi.org/10.1385/ABAB:79:1-3:681

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