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Construction of recombinant Bacillus subtilis for production of polyhydroxyalkanoates

  • Session 6 Bioprocess Research and Development
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Abstract

Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyalkanoates synthesized by numerous bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. In this work, we constructed two recombinant plasmids, pBE2C1, and pBE2C1AB, containing one or two PHA synthse, genes, respectively. The two plasmids were inserted into Bacillus subtilis DB104 to generate modified strains, B. subtilis/pBE2C1 and B. subtilis/pBE2C1AB. The two recombinants strains were subjected to fermentation and showed PHA accumulation, the first reported example of mcl-PHA production in B. subtilis. Gas Chromatography analysis identified the compound produced by B. subtilis/pBE2C1 to be a hydroxydecanoate-co-hydroxydodecanoate (HD-co-HDD) polymer whereas that produced by B. subtilis/pBE2C1AB was a hydroxybutyrate-co-hydroxyde-canoate-co-hydroxydodecanoate (HB-HD-HDD) polymer.

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Authors and Affiliations

  1. Department of Applied Biology and Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, China

    Yujie Wang, Lifang Ruan, Wai-Hung Lo & Hoi-Fu Yu

  2. Department of Civil and Structural Engineering, The Hong Kong Polytechnic University, Hong Kong, China

    Hong Chua

Authors
  1. Yujie Wang
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  2. Lifang Ruan
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  3. Wai-Hung Lo
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  4. Hong Chua
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  5. Hoi-Fu Yu
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Correspondence to Hoi-Fu Yu.

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Wang, Y., Ruan, L., Lo, WH. et al. Construction of recombinant Bacillus subtilis for production of polyhydroxyalkanoates. Appl Biochem Biotechnol 132, 1015–1022 (2006). https://doi.org/10.1385/ABAB:132:1:1015

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  • DOI: https://doi.org/10.1385/ABAB:132:1:1015

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