Abstract
The water-soluble portion and precipitates obtained by supercritical (SC) water treatment of microcrystalline cellulose (Avicel) were enzymatically hydrolyzed. Glucose could be produced easily from both substrates, compared with the Avicel. Therefore, SC water treatment was found to be effective for enhancing the productivity of glucose from cellulose by the enzymatic hydrolysis. It is also found that alkaline treatment or wood charcoal treatment reduced inhibitory effects by various decomposed compounds of cellulose on the enzymatic hydrolysis to achieve higher glucose yields. Furthermore, glucose obtained by SC water treatment followed by the enzymatic hydrolysis of cellulose could be converted to ethanol by fermentation without any inhibition.
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References
Yu, Z. and Zhang, H. (2004), Biores. Technol., 93, 199–204.
Söderström, M., Pilcher, L., Galbe, M., and Zacchi, G. (2003), Biomass. Bioenergy 24, 475–486.
Cantarella, M., Cantarella, L., Gallifuoco, A., Spera, A., and Alfani, F. (2003), Biotechnol. Prog. 20, 200–206.
Laser, M., Schulman, D., Allen, S. G., Lichwa, J., Antal Jr. M. J., and Lynd, L. R. (2002), Biores. Technol. 81, 33–44.
Ortega, N., Busto, M. D., and Perez-Maeteos, M. (2002), Biomass Bioenergy 47, 7–14.
Ueno, T. and Saka, S. (1999), Cellulose 6, 177–191.
Saka, S. and Konishi, R. (2001), In: Progress in Thermochemical Biomass Conversion, Bridgwater, A.V., ed., Blackwell Science, Oxford, pp. 1338–1348.
Iahikawa, Y. and Saka, S. (2001), Cellulose, 8, 189–195.
Ehara, K., Saka, S., and Kawamoto, H. (2002), J. Wood Sci. 48, 320–325.
Tsujino, J., Kawamoto, H., and Saka, S. (2003), Wood Sci. Technol. 37, 299–307.
Minami, E. and Saka, S. (2003), J. Wood Sci. 49, 73–78.
Minami, E., Kawamoto, H., and Saka, S. (2003), J. Wood Sci. 49, 158–165.
Ehara, K. and Saka, S. (2002), Cellulose 9, 301–311.
Takada, D., Ehara, K., and Saka, S. (2004), J. Wood Sci. 50, 253–259.
Yoshida, K., Ehara, K., and Saka, S. (2005), Appl. Biochem. Biotechnol. 121–124, 795–806.
Miyafuji, H., Nakata, T., Ehara, K., and Saka, S. (2005), Appl. Biochem. Biotechnol. 121–124, 963–971.
Sasaki, M., Iwasaki, K., Hamaya, T., Adschiri, T., and Arai, K. (2001), Koubunshi Ronbunshu 58, 527–532.
Miyafuji, H., Danner, H., Neureiter, M., Thomasser, C., and Braun, R. (2003), Biotechnol. Bioeng. 84, 390–393.
Miyafuji, H., Danner, H., Neureiter, M., et al. (2003), Enzyme Microb. Technol. 32, 396–400.
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Nakata, T., Miyafuji, H. & Saka, S. Bioethanol from cellulose with supercritical water treatment followed by enzymatic hydrolysis. Appl Biochem Biotechnol 130, 476–485 (2006). https://doi.org/10.1385/ABAB:130:1:476
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DOI: https://doi.org/10.1385/ABAB:130:1:476