Abstract
Microencapsulated Aspergillus niger with mycelium-bound tannase activity was employed to investigate the esterification of propyl gallate from gallic acid and propanol in organic solvents. The effects of various organic solvents (log P:−1.0 to 6.6) on the enzymatic reactions showed that benzene (log P:2.0) was the suitable solvent, for which the conversion reached 26.8%. The optimum catalyst concentration and water concentration was found at 25 capsules in 10 mL of benzene and 0.04 g of water/capsule. The external mass transfer effect could be eliminated at stirring speeds of 180 rpm or higher. Both substrates 1-propanol and gallic acid had significant inhibition effects on the tannase activity. Maximum molar conversion (36.2%) was achieved with 9.1% (v/v) 1-propanol and 8 mM gallic acid and decreased with increasing amounts of substrates.
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Yu, XW., Li, YQ. Microencapsulated mycelium-bound tannase from Aspergillus niger . Appl Biochem Biotechnol 126, 177–187 (2005). https://doi.org/10.1385/ABAB:126:3:177
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DOI: https://doi.org/10.1385/ABAB:126:3:177