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Effect of process parameters on production of a biopolymer by Rhizobium sp.

  • Session 3—Bioprocessing, Including Separations
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Abstract

The production of biopolymers by a Rhizobium strain was studied under batch and bioreactor conditions. The best viscosity levels were obtained under low mannitol concentrations as well as low agitation and aeration conditions. Infrared spectra indicated the presence of chemical groups characteristic of microbially produced biopolymers, including C=O and O-acetyl groups. Thermogravimetric analysis showed the characteristic degradation profiles of the exopolysaccharide produced (T onset=290°C). The experimental design showed that a low substrate concentration (10.0 g/L), and low aeration (0.2 vvm) and agitation (200 rpm) levels should be used. The maximum yield of the process was a Yp/s (g/g) of 0.19±0.1, obtained under optimized conditions.

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Authors and Affiliations

  1. Departamento de Engenharia Bioquímica, Bloco E, Ilha do Fundão, Universidade Federal do Rio de Janeiro, CEP: 21949-900, Rio de Janeiro, RJ, Brazil

    Flávia Pereira Duta, Francisca Pessôa De França & Eliana Flávia Camporese Sérvulo

  2. Instituto de Macromoléculas Professora Eloisa Mano, Rio de Janeiro-RJ, Brazil

    Léa Maria De Almeida Lopes

  3. Departamento de Tecnologia de Processos Bioquímicos, Universidade do Estado do Rio de Janeiro, Portugal

    Antonio Carlos Augusto Da Costa

  4. Universidade de Aveiro, Portugal

    Ana Barros

Authors
  1. Flávia Pereira Duta
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  2. Francisca Pessôa De França
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  3. Eliana Flávia Camporese Sérvulo
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  4. Léa Maria De Almeida Lopes
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  5. Antonio Carlos Augusto Da Costa
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  6. Ana Barros
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Correspondence to Francisca Pessôa De França.

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Duta, F.P., De França, F.P., Sérvulo, E.F.C. et al. Effect of process parameters on production of a biopolymer by Rhizobium sp.. Appl Biochem Biotechnol 114, 639–652 (2004). https://doi.org/10.1385/ABAB:114:1-3:639

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  • DOI: https://doi.org/10.1385/ABAB:114:1-3:639

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