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4-Chlorophenol degradation by chloroperoxidase from Caldariomyces fumago

Formation of insoluble products

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Abstract

This study investigated the degradation of 4-chlorophenol (4-CP) by Caldariomyces fumago chloroperoxidase (CPO). Enzymatic oxidations were studied in reaction mixtures at pH 3.0, 4.0, and 6.0 in the presence and absence of Cl− containing 3.5 IU of CPO and 4-CP and hydrogen peroxide concentrations within the range of 0.5–50 and 0.005–50 mM, respectively. Distinct patterns of products regarding color, concentration, and solubility were observed. Reaction mixtures at pH 6.0 containing 3.5 IU of CPO and 5.0 mM 4-CP and H2O2 (1:1 stoichiometry) showed the highest 4-CP removal of 95% and the highest formation of a dark precipitate.

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Authors and Affiliations

  1. Laboratório de Tecnologia Enzimática, Instituto de Química, Universidade Federal do Rio de Janeiro, CT, Bloco A, Ilha do Fundão, 21949-900, Rio de Janeiro, RJ, Brasil

    Camilo E. La Rotta H. & Elba P.S.Bon

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  1. Camilo E. La Rotta H.
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  2. Elba P.S.Bon
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Correspondence to Elba P.S.Bon.

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La Rotta H., C.E., P.S.Bon, E. 4-Chlorophenol degradation by chloroperoxidase from Caldariomyces fumago . Appl Biochem Biotechnol 98, 191–203 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:191

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  • DOI: https://doi.org/10.1385/ABAB:98-100:1-9:191

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