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Study on methane fermentation and production of vitamin B12 from alcohol waste slurry

  • Session 6A Biomass Pretreatment and Hydrolysis
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Abstract

We studied biogas fermentation from alcohol waste fluid to evaluate the anaerobic digestion process and the production of vitamin B12 as a byproduct. Anaerobic digestion using acclimated methanogens was performed using the continuously stirred tank reactor (CSTR) and fixed-bed reactor packed with rock wool as carrier material at 55°C. We also studied the effects of metal ions added to the culture broth on methane and vitamin B12 formation. Vitamin B12 production was 2.92 mg/L in the broth of the fixed-bed reactor, twice that of the CSTR. The optimum concentrations of trace metal ions added to the culture liquid for methane and vitamin B12 production were 1.0 and 8 mL/L for the CSTR and fixed-bed reactor, respectively. Furthermore, an effective method for extracting and purifying vitamin B12 from digested fluid was developed.

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Authors and Affiliations

  1. Institute of Agricultural and Forest Engineering, University of Tsukuba, 1-1-1 Tennodai, 305-8573, Tsukuba, Ibaraki, Japan

    Zhenya Zhang, Norio Sugiura & Takaaki Maekawa

  2. Mater Program of Biosystem Studies, University of Tsukuba, 1-1-1 Tennodai, 305-8573, Tsukuba, Ibaraki, Japan

    Taisheng Quan

  3. Doctoral Program of Agricultural Science, University of Tsukuba, 1-1-1 Tennodai, 305-8573, Tsukuba, Ibaraki, Japan

    Pomin Li

  4. Water Conservancy and Civil Engineering College, China Agricultural University, Peking, Haidian District, Qinhua Dong Road, China

    Yansheng Zhang

Authors
  1. Zhenya Zhang
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  2. Taisheng Quan
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  3. Pomin Li
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  4. Yansheng Zhang
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  5. Norio Sugiura
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  6. Takaaki Maekawa
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Correspondence to Zhenya Zhang.

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Zhang, Z., Quan, T., Li, P. et al. Study on methane fermentation and production of vitamin B12 from alcohol waste slurry. Appl Biochem Biotechnol 115, 1033–1039 (2004). https://doi.org/10.1385/ABAB:115:1-3:1033

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  • DOI: https://doi.org/10.1385/ABAB:115:1-3:1033

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