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Preparation and Characterization of a Microwave-Immobilized Fluorinated Stationary Phase for RP-LC

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Abstract

A fluorinated stationary phase was prepared through the immobilization of poly(methyl-3,3,3-trifluoropropylsiloxane) onto 5 μm Kromasil silica by microwave irradiation. The best conditions of immobilization time and temperature were determined by central composite design and response surface methodology. Physical–chemical characterizations (IR, 29Si NMR and elemental analysis) confirmed that the polymer was attached onto the chromatographic support by different mechanisms that resulted in a percent carbon loading of 10%. Some pharmaceuticals were completely separated with the fluorinated stationary phase using a simple mobile phase while the same separation was not possible with a C18 stationary phase.

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Acknowledgments

The authors acknowledge financial support and fellowships from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The authors also thank C.H. Collins for helpful discussions and suggestions.

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Authors and Affiliations

  1. Institute of Chemistry, University of Campinas, P.O. Box 6154, 13083-970, Campinas, SP, Brazil

    Liane Maldaner & Isabel C. S. F. Jardim

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  1. Liane Maldaner
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  2. Isabel C. S. F. Jardim
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Correspondence to Isabel C. S. F. Jardim.

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Maldaner, L., Jardim, I.C.S.F. Preparation and Characterization of a Microwave-Immobilized Fluorinated Stationary Phase for RP-LC. Chroma 72, 617–626 (2010). https://doi.org/10.1365/s10337-010-1726-3

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