Skip to main content
SpringerLink
Log in

Preparation of branched-polyfluoroalkylsilane-coated silica gel columns and their HPLC separation characteristics

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

The conditions for reaction of the branched polyfluoroalkylsilane D3CL with silica gel have been examined. From elemental analyses and29Si NMR spectra it was found that the surface coverage and endcapping of D3CL-coated silica gel (Fluofix®) were the same as for ODS packings.

The separation behavior of these phases was compared that of a variety of other packings. For polyaromatic compounds and benzene derivatives Fluofix worked in the reversed-phase mode and its retention characteristics were similar to those of C1 or CN packings. For fluorinated compound the retention characteristics of the phases is similar to those of ODS although with MeOH-water and MeCN-water mobile phases the pressure loss was lower for the fluorinated phases than for ODS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. Varughese, M. E. Gangoda, R. K. Gilpin, J. Chromatogr. Sci.26, 401 (1988).

    CAS  Google Scholar 

  2. N. D. Danielson, L. G. Beaver, J. Wangsa, J. Chromatogr.544, 187 (1991).

    Article  CAS  Google Scholar 

  3. M. E. Gangoda, R. K. Gilpin, J. Chromatogr.448, 365 (1988).

    Article  CAS  Google Scholar 

  4. R. M. Pomaville, C. F. Poole, Anal. Chem. Acta.200, 151 (1987).

    Article  CAS  Google Scholar 

  5. D. A. Usher, Nucleic Acids Res.6, 2289 (1979).

    CAS  Google Scholar 

  6. R. W. Siergiej, N. D. Danielson, Anal. Chem.55, 17 (1983).

    CAS  Google Scholar 

  7. H. Sawai, J. Chromatogr.481, 201 (1989).

    Article  CAS  Google Scholar 

  8. S. Hjertén, J. Chromatogr.159, 47 (1978).

    Article  Google Scholar 

  9. R. C. Williams, J. F. Vasta-Rusell, J. L. Glajch, K. Golebiowski, J. Chromatogr.371, 63 (1986).

    Article  CAS  Google Scholar 

  10. C. Hirayama, H. Ihara, S. Nagaoka, K. Hamada, J. Chromatogr.465, 241 (1989),C. Hirayama, Ekitaikuromatogurafiyo Porimajutenzairyou no Kaihatsugijutsu, IPC, Tokyo, 1992, p. 238;C. Hirayama, Y. Okamoto, Kuromatoyojushi, Kyoritsu Shuppan, Tokyo, 1989, p. 47.

    Article  CAS  Google Scholar 

  11. G. E. Berendsen, K. A. Pikaart, L. de Galan, C. Olieman, Anal. Chem.52, 1990 (1980).

    Article  CAS  Google Scholar 

  12. H. A. H. Billet, P. J. Schoenmakers, L. de Galan, J. Chromatogr.218, 455 (1981).

    Article  Google Scholar 

  13. W. Li, J. Wang, C. A., 96, 52376 (1982).

    Google Scholar 

  14. J. J. Lewis, L. B. Rogers, R. E. Pauls, J. Chromatogr.264, 339 (1983).

    Article  CAS  Google Scholar 

  15. J. J. Lewis, L. B. Rogers, R. E. Pauls, J. Chromatogr.299, 331 (1984).

    Article  CAS  Google Scholar 

  16. A. Hass, J. Kohler, H. Hemetsberger, Chromatographia14, 341 (1981).

    Article  Google Scholar 

  17. P. C. Sadek, P. W. Carr, J. Chromatogr.288, 25 (1984).

    Article  CAS  Google Scholar 

  18. P. C. Sadek, P. W. Carr, M. J. Ruggio, Anal. Chem.59, 1032 (1987).

    Article  CAS  Google Scholar 

  19. M.-P. Krafft, F. Jeannaux, M. LeBlanc, J. G. Reiss, A. Berthod, Anal Chem.60, 1969 (1988).

    Article  CAS  Google Scholar 

  20. G. Xindi, P. W. Carr, J. Chromatogr.269, 96 (1983).

    Article  Google Scholar 

  21. F. M. Rabel, E. T. Buuts, LC-GC,1, 42 (1983).

    CAS  Google Scholar 

  22. E. Csato, N. Fulop, G. Szabo, J. Chromatogr.511, 79 (1990).

    Article  CAS  Google Scholar 

  23. G. Felix, C. Bartrand, J. High Resol. Chromatogr. Chromatogr. Commun.9, 411 (1987).

    Article  Google Scholar 

  24. W. Ecknig, B. Trung, R. Radeglia, U. Gross, Chromatographia16, 178 (1982).

    Article  CAS  Google Scholar 

  25. G. Felix, A. Thienpont, C. Bartrand, Chromatographia23, 684 (1987).

    Article  CAS  Google Scholar 

  26. G. Felix, C. Bartrand, J. High Resol. Chromatogr. Chromatogr. Commun.8, 362 (1985).

    Article  CAS  Google Scholar 

  27. J. Kip, P. van Haperen, J. C. Kraak, J. Chromatogr.356, 423 (1986).

    Article  CAS  Google Scholar 

  28. N. D. Danielson, S. A. Shamsi, S. A. Maki, J. High Resol. Chromatogr. Chromatogr. Commun.15, 343 (1992).

    Article  CAS  Google Scholar 

  29. D. W. William, P. M. Kabra, Anal. Chem.62, 807 (1990).

    Article  Google Scholar 

  30. T. Konakahara, S. Okada, T. Monde, N. Nakayama, J. Furuhashi, J. Sugaya, Nippon Kagaku Kaishi1991, 1638 (1991).

    Google Scholar 

  31. H. Itoh, Toshio Kinoshita, Nobuyuki Nimura, J. Chromatogr. A662, 95 (1994).

    Article  CAS  Google Scholar 

  32. K. Jinno, H. Nakamura, Chromatographia39, 285 (1994).

    Article  CAS  Google Scholar 

  33. T. Monde, T. Kamiusuki, T. Kuroda, K. Mikumo, T. Ohkawa, H. Fukube, J. Chromatogr. A,722, 273 (1996);T. Monde, K. Kamiusuki, T. Kuroda, Fine Chemicals24, 31 (1995).

    Article  CAS  Google Scholar 

  34. T. Monde, T. Kamiusuki, T. Kuroda, K. Mikumo, H. Hatano, Pittcon ,93 Abstract No.76P, 458P, Atlanta, GA, USA, March 5–10, 1993,T. Kamiusuki, T. Kuroda, K. Mikumo, T. Monde, H. Fukube, B. Z. Tang, Pittcon ,94 Abstract No.21P, Chicago, IL, USA, February 27–March 4, 1994,T. Monde, T. Kamiusuki, T. Kuroda, K. Mikumo, T. Ohkawa, H. Fukube, B. Z. Tang, Pittcon ,95 Abstract No.76P, New Orleans, LA, USA, March 5–10, 1995,T. Monde, T. Kamiusuki, T. Kuroda, K. Mikumo, T. Ohkawa, H. Fukube, Int. Symp. Chromatogr., Abstract p267, Yokohama, Japan, January 22–25, 1995,H. Hatano, T. Monde, T. Kamiusuki, T. Kuroda, K. Mikumo, H. Fukube, Int. Symp. Fluorine Chem., Abstract p53, Yokohama, Japan, July 31–August 5, 1994,T. Yuno, T. Monde, T. Kamiusuki, K. Mikumo, H. Kohama, T. Konakahara, Int. Conf. High Technology, Conf. Proc., p155, Chiba, Japan, September 18–19, 1992.

  35. S. Brunauer, P. H. Emmett, E. Teller, J. Am. Chem. Soc.60, 309 (1938).

    Article  CAS  Google Scholar 

  36. E. P. Barrett, L. G. Joyner, P. P. Halenda, J. Am. Chem. Soc.73, 373 (1951).

    Article  CAS  Google Scholar 

  37. P. C. Sadek, P. W. Carr, J. Chromatogr.288, 25 (1984);G. E. Berendsen, K. A. Pikaart, L. de Galan, Anal. Chem.52, 1990 (1980);H. A. H. Billiet, P. J. Schoenmakers, L. de Galan, J. Chromatogr.218, 443 (1981);K. Jinno, S. Shimura, N. Tanaka, K. Kimata, J. C. Fetzer, W. R. Biggs, Chromatographia27, 285 (1989).

    Article  CAS  Google Scholar 

  38. D. W. Sindorf, G. E. Maciel, J. Am. Chem. Soc.105, 1487 (1983).

    Article  CAS  Google Scholar 

  39. J. Köhler, D. B. Chase, R. D. Farlee, A. J. Vega, J. J. Kirkland, J. Chromatogr.352, 275 (1986).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central Research Laboratory, NEOS Company Ltd, 520-3213, Shiga, Japan

    T. Kamiusuki, T. Monde & K. Yano

  2. Institute for Chemical Research, Kyoto University, 611-0011, Kyoto, Japan

    T. Yoko

  3. Faculty of Science and Technology, Science University of Tokyo, 278-8510, Chiba, Japan

    T. Konakahara

Authors
  1. T. Kamiusuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Monde
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Yano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Yoko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Konakahara
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kamiusuki, T., Monde, T., Yano, K. et al. Preparation of branched-polyfluoroalkylsilane-coated silica gel columns and their HPLC separation characteristics. Chromatographia 49, 649–656 (1999). https://doi.org/10.1007/BF02466907

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02466907

Key Words

Search

Navigation