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LC-UV Column-Switching for Quantitation of Vitamin K1 in Infant Formula

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Abstract

This study was undertaken in order to develop an analytical method for vitamin K1 in infant formula. The content of vitamin K1 was investigated by using a column-switching LC-UV method. A Certified Reference Material sample of infant formula containing 0.94 ± 0.04 mg kg−1 of vitamin K1 was extracted with hexane followed by enzymatic digestion of fat and precipitation of the fatty acids. The linearity of this method was calculated using five consecutive standard curves, and the coefficient of determination (r 2) was found to be 0.9995. The limit of detection (LOD) and the limit of quantitation (LOQ) was 3.31 and 11.12 μg L−1, respectively. The accuracy of intra- and inter-day measurements was in the range from 96.67 to 108.67%, and the precision of intra- and inter-day measurements was less than 5.13%. The recoveries were 109.27 ± 5.92%, and the recoveries of inter-laboratory results were in the range from 97.59 ± 1.29 to 109.27 ± 5.92%. The newly developed method uses the optimum conditions required to determine the content of vitamin K1 in infant formula.

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Acknowledgments

We are grateful to the Korea Food and Drug Administration research project (KFDA 09081KFDA088) which provided financial support for this study in 2009. We would also like to thank Dr. Jayoung Jeong, Dr. Leem Dong-Gil and Mr. Taehyung Yoon of the KFDA Nutrition and Functional Food Research Team. In addition, we thank Prof. Kyong-Su Kim of Chosun University for his help as a project partner.

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Authors and Affiliations

  1. Food Safety Center, Research and Development Institute, Namyang Dariy Co. Ltd, Bongan-Ri 160, Janggi-Myeon, Gongju, 314-914, Republic of Korea

    Jinhyouk Ko, Byung-Man Kwak, Jang-Hyuk Ahn & Seung-Hwan Jeong

  2. Department of Food and Nutrition, Chosun University, 375 Seo-suk dong, Dong-gu, Kwangju, 501-759, Republic of Korea

    Sung-Lye Shim & Kyong-Su Kim

  3. Nutrition and Functional Food Research Team, Korea Food and Drug Administration, 194 Tongil-ro, Eunpyung-gu, Seoul, 122-704, Republic of Korea

    Taehyung Yoon, Leem Dong-gil & Jayoung Jeong

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  1. Jinhyouk Ko
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  2. Byung-Man Kwak
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Correspondence to Jang-Hyuk Ahn.

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Ko, J., Kwak, BM., Ahn, JH. et al. LC-UV Column-Switching for Quantitation of Vitamin K1 in Infant Formula. Chroma 72, 665–670 (2010). https://doi.org/10.1365/s10337-010-1722-7

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