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LC Determination of Peroxynitrite Scavenging Activity of Phenols from Salvia spp.

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Abstract

The aim of this study was to adopt pyrogallol red bleaching test for LC-DAD capable of characterizing chemical constituents and peroxynitrite (ONOO−) scavenging activity in parallel for a large number of plant extracts. The hypothesis is that upon reaction with ONOO−, the peak areas of compounds in complex mixtures with potential radical scavenging activity in the LC chromatograms will be significantly reduced or disappeared. We validated this approach with a model mixture of 17 phenolic compounds, which mimics a general alcoholic extract of Salvia species. The LC separation conditions were optimized for baseline separation of phenolic reference compounds and for selective detection of the test substrate pyrogallol red. The results indicated that the depletion kinetic of compounds in the model mixture correlates moderately with the individual scavenging activities. Moreover, catechol group dependent relationship between the structure–scavenging activity relationships confirmed the LC based assay parameters. The final demonstration of the assay was by methanolic extract of Salvia miltiorrhiza Bunge, which showed outstanding ONOO− scavenging activity. 13 min total analysis time per sample and excellent resolution allow the method to be applied for chemical fingerprinting coupled with rapid screening for natural antioxidants derived from alcoholic extracts of Salvia species.

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Acknowledgments

The authors wish to thank Professor Imre Máthé, University of Szeged Faculty of Pharmacy, Department of Pharmacognosy for providing S. miltiorrhiza samples for our research.

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Authors and Affiliations

  1. Discovery Chemistry, Gedeon Richter Plc., Gyömrői u. 19-21., Budapest, 1475, Hungary

    Árpád Könczöl, György Miklós Keserű & György Tibor Balogh

  2. Department of Pharmacognosy, Semmelweis University, Üllői u. 26., Budapest, 1085, Hungary

    Ágnes Kéry

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  1. Árpád Könczöl
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  2. Ágnes Kéry
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  3. György Miklós Keserű
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  4. György Tibor Balogh
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Correspondence to György Tibor Balogh.

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Könczöl, Á., Kéry, Á., Keserű, G.M. et al. LC Determination of Peroxynitrite Scavenging Activity of Phenols from Salvia spp.. Chroma 71 (Suppl 1), 51–59 (2010). https://doi.org/10.1365/s10337-010-1528-7

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