Abstract
Sugars present in medicinal plants are known for protecting and stimulating the immune system against various biological disorders. Tinospora cordifolia is a reputed Indian herb used for immunity enhancing which is mainly attributed to saccharides. In the present study, a simple, sensitive, and reliable liquid chromatography method based on ultrasonic assisted extraction and evaporative light scattering detection was developed for simultaneous determination of ten sugars comprising of monosaccharides (l-(+)-rhamnose, d-(+)-xylose, d-(−)-arabinose, β-d-(+)-glucose), disaccharides (sucrose, d-(+)-cellobiose, α-lactose), alditols (xylitol, d-(+)-mannitol) and a polyalcohol (myo-inositol) in T. cordifolia. The separation was achieved on Zorbax-NH2 column (250 mm × 4.6 mm, 5 µm) in gradient elution of acetonitrile: water as mobile phase with flow rate of 0.5 mL min−1. The drift tube temperature and nitrogen flow-rate were optimized at 70 °C and 2.0 standard litres per minute, respectively. The method was validated for linearity, accuracy, precision, limits of detection and quantification. The calibration equation revealed a good linear relationship (r 2 = 0.959–0.999). The sufficient recovery was observed in the range of 94.1–99.9%. The method showed good reproducibility with intra- and inter-day precision of <0.99 and 0.97% (RSD), respectively. The detection and quantification limits for the compounds were in the range of 8.32–44.29 and 25.23–134.20 μg mL−1, respectively.
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Acknowledgments
Authors are grateful to the director IHBT, Palampur for providing necessary facilities during the work. Financial support from the Department of Biotechnology, Government of India is highly acknowledged. Upendra sharma and Pamita Bhandari are also grateful to CSIR for awarding Research Fellowship.
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Sharma, U., Bhandari, P., Kumar, N. et al. Simultaneous Determination of Ten Sugars in Tinospora cordifolia by Ultrasonic Assisted Extraction and LC-ELSD. Chroma 71, 633–638 (2010). https://doi.org/10.1365/s10337-010-1520-2
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DOI: https://doi.org/10.1365/s10337-010-1520-2