Summary
This study is a first attempt to present a report about a high-performance thin-layer chromatography (HPTLC)—densitometric method that has been developed and validated for the quantification of sugars, i.e., glucose, fructose, sucrose, maltose, and raffinose from the methanol extracts of roots of 7 Asparagus species — A. adscendens, A. racemosus, A. retrofractus, A. officinalis, A. densiflorus, A. falcatus, and A. sprengeri. The separation and quantification of sugars were achieved using the mobile phase propanolethyl acetate — water (6:3:1, v/v). Derivatization of the bands was done by using diphenylamine, aniline, and ortho-phosphoric acid reagents, and densitometric scanning was done at 600 nm in absorbance—reflectance mode. This method gave the best resolution of bands at RF = 0.55, 0.50, 0.31, 0.11, and 0.46, corresponding to fructose, glucose, maltose, raffinose, and sucrose, respectively. The calibration curves were linear in the range of 100–500 ng per spot. The limit of detection (LOD) and the limit of quantification (LOQ) ranged between 12.77–75.48 ng and 38.69–228.73 ng, respectively. The intra-and inter-day precisions were found between 0.33–1.46 in terms of relative standard deviation (RSD). The average recoveries ranged between 97.84–99.91%. The validated method was successfully used to detect and quantify sugars in the methanol root extracts of the 7 Asparagus species.
Similar content being viewed by others
References
S. Velaven, K. Nagulendran, R. Mahesh, V.H. Begum Pharmacogn. Rev., 1 (2007) 350–360.
A. Kanno, J. Yokoyama, in: C. Kole (ed.), Wild Crop Relatives: Genomic and Breeding Resources – Vegetables, Springer, New York, NY, 2011, pp. 23–42.
K.D. Prabakaran, R. Vadivu, N. Jayshree I nt. J. Multidiscip. Res. Dev., 2 (2015) 332–335.
A. Singh, B. Sinha Asian J. Biochem. Pharm. Res., 4 (2014) 230–240.
J.S. Negi, P. Singh, G.P. Joshi, M.S. Rawat, V.K. Bisht Pharmacogn. Rev., 4 (2010) 215–220.
A. Singh, V.P. Rai, M. Singh, A.K. Singh, B. Sinha J. Med. Plants Res., 7 (2013) 1050–1056.
P.Y. Hayes, A.H. Jahidin, R. Lehmann, K. Penman, W. Kitching, J.J. De Voss Tetrahedron Lett., 47 (2006) 8683–8687.
N. Shiomi New Phytol., 122 (1992) 421–432.
N. Benkeblia, N. Yoshida, Y. Ooi, T. Nagamine, S. Onodera, N. Shiomi Acta Horti., 776 (2008) 459–464.
W.E. Cake, H.H. Bartlett J. Biol. Chem., 51 (1922) 93–102.
W. Krzesiński, M. Gqsecka, J. Stachowiak, M. Knafl ewski, Folia Horti. 20/2 (2008a) 29–38.
W. Krzesiński, M. Gqsecka, J. Stachowiak, A. Zurawicz, M. Knaflewski, P. Goliński, Acta Sci. Pol. Hortorum Cultus 7 (2008b) 93–102.
A. Aberoumand Nutr. Food Sci. Res., 1 (2014) 57–61.
E. Pressman, A. Schaff er, D. Compton, E. Zamski J. Plant Physiol., 143 (1994) 621–624.
P.K. Bhowmik, T. Matsui Asian J. Plant Sci., 2 (2003) 891–893.
M. Gqsecka, J. Stachowiak, W. Krzesiński, M. Knafl ewski, P. Goliński Veg. Crops Res. Bull., 69 (2008) 145–154.
R.P. Hewawasam, K.A.P.W. Jayatilaka, C. Pathirana J. Diet. Suppl., 5 (2018) 1–19.
I. Khan, M. Nisar, N. Khan, M. Saeed, S. Nadeem, F. Rehman, F. Ali, N. Karim, W.A. Kaleem, M. Qayum, H. Ahmad, I.A. Khan Fitoterapia, 81 (2010) 1020–1025.
R.M. Ghalib, R. Hashim, O. Sulaiman, S.H. Mehdi, A. Valkonen, K. Rissanen, S.R. Trifunović, M.B.K. Ahamed, A.M.S.A. Majid, F. Kawamura Eur. J. Med. Chem., 47 (2012) 601–607.
P. Pahwa, R.K. Goel Chem. Biol. Interact., 260 (2016) 208–218.
KM. Khan, L. Nahar, A. Mannan, M. Arfan, G.A. Khan, A. Al-Groshi, A. Evans, N.M. Dempster, F.M.D. Ismail, S.D. Sarker Pharmacogn. Mag., 13 (2017) S890–S894.
Validation of analytical procedure: methodology Q2 (R1), International Conference on Harmonization (ICH), International Federation of Pharmaceutical Manufacturers and Associations (IFPMA), Geneva, 1996.
L. Dinan, T. Savchenko, P. Whiting Phytochemistry, 56 (2001) 569–576.
J.M.F. Alventosa, J.M.M. Rojas, in: V., Preedy (ed.) Processing and Impact on Active Components in Food, Elsevier/Academic Press, Amsterdam, Boston, 2015, pp. 103–110.
Q. Zhao, B. Xie, J. Yan, F. Zhao, J. Xiao, L. Yao, B. Zhao, Y. Huang Carbohydr. Polym., 87 (2012) 392–396.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kapoor, M., Gupta, R.C., Mawal, P. et al. Determination of Sugar Biomarkers in Methanol Root Extracts of Asparagus Species by High-Performance Thin-Layer Chromatography. JPC-J Planar Chromat 32, 109–114 (2019). https://doi.org/10.1556/1006.2019.32.2.5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/1006.2019.32.2.5