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Characterization of Convection for Molecularly Imprinted Monolith

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Abstract

Convection of molecularly imprinted polymers monolith in LC mode was discussed in this paper. On the MIPs monolith reported here, a flat van Deemter plot of height equivalent to a theoretical plate (HETP) versus superficial velocity was observed. This typical behavior, similar to perfusion packings, suggests that the unique pore structure of the MIPs monolith allowed convection-enhanced mass transfer. Column parameters, e.g., external porosities, internal porosity, column permeability and equivalent sphere dimension, were obtained. Intraparticle Peclet number (λ) was used to characterize the convection in the monolith. In addition, a ratio of the numbers of transfer units, T, for diffusion in the micropores and through-pores has been introduced to quantify the relative importance of the contribution from convection and diffusion to mass transfer. The results show that the flow in a MIP monolith is extremely sensitive to pore size distribution and can be tuned by polymerization parameters.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 20575045) and Natural Science Foundation of Tianjin (No. 08JCYBJC02000).

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Authors and Affiliations

  1. College of Pharmacy, Tianjin Medical University, 300070, Tianjin, China

    Yan-Ping Huang, Shu-Jing Zhang, Liang Zhao, Qin-Wei Zhang & Zhao-Sheng Liu

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  1. Yan-Ping Huang
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  2. Shu-Jing Zhang
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  3. Liang Zhao
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  4. Qin-Wei Zhang
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Corresponding author

Correspondence to Zhao-Sheng Liu.

Glossary

B b

Column permeability

B p

Particle permeability

b

Retention parameter defined with 1 + (1 − ε p) m/ε p

C

Experimental C-term mass transfer parameter in van Deemter equation

D

Effective pore diffusivity

D c

Effective diffusivity in micropore

D eff

Effective diffusivity in throughpores

D em

Effective diffusivity in the microparticle pores

D m

Molecular diffusivity

d pore

Equivalent pore size

d m

Diameter of the gel microspheres

d disp

Equivalent sphere dimensions

m

Retention parameter defined in Eq.  14

K

Equilibrium distribution coefficient

K′

Adsorption factor

k

Boltzmann constant

k′

Capacity factor

L

Column length

l

Half-thickness of the slab

M

Molecular weight of the solvent

n m

Mass transfer unit for diffusion in micropores

n t

Mass transfer unit for diffusion in throughpores

ΔP

Pressure drop

Q

Volume flowrate

r p

Half-thickness of the slab in bidispersed pore model

r c

Radius of micropores \( \left( { = {\frac{1}{2}}d_{\text{m}} } \right) \)

r

Molecular radius

T

Ratio of the numbers of transfer units for diffusion in the microparticles and throughpores

t 0

Retention time of a unretained substance, thiourea

t d

Retention time of very large molecules, blue dextran

u 0

Bed superficial velocity

V m

Molar volume of the adsorbate

Greek letters

 

α

Split ratio

β

Phase ratio

γ

Internal tortuosity factor

ε e

External porosity

ε p

Internal porosity

ε t

Total porosity

ε m

Porosity of microparticle

η

Viscosity of the mobile phase

λ

Intraparticle Peclet number

v 0

Pore flow velocity

ϕ

Constant that accounts for solute–solvent interactions

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Huang, YP., Zhang, SJ., Zhao, L. et al. Characterization of Convection for Molecularly Imprinted Monolith. Chroma 71, 559–569 (2010). https://doi.org/10.1365/s10337-010-1513-1

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  • DOI: https://doi.org/10.1365/s10337-010-1513-1

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