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Simultaneous Separation and Determination of Isosorbide Dinitrate and Isosorbide 5-Mononitrate in Human Plasma by LC–MS–MS

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Abstract

A sensitive, selective, and simple liquid chromatography-tandem mass spectrometry method has been developed for the simultaneous separation and determination of isosorbide dinitrate and its active metabolite, isosorbide 5-mononitrate, in human plasma. Topiramate was used as the internal standard. Sample preparation consisted of a simple one-step liquid–liquid extraction with ethylacetate without pH adjustment. The method was fully validated with respect to linearity, sensitivity, specificity, recovery, accuracy, and precision. Isosorbide dinitrate and isosorbide 5-mononitrate were stable in standard solution and in plasma samples under storage and processing conditions. The assay was successfully applied to the pharmacokinetic study of isosorbide dinitrate and isosorbide 5-mononitrate in human plasma.

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Acknowledgments

This work was supported by a Research Grant from Chung-Ang University (2008).

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Authors and Affiliations

  1. College of Pharmacy, Chung-Ang University, Seoul, 156-756, South Korea

    Hohyun Kim & Sang Beom Han

  2. College of Pharmacy, Kangwon National University, Chuncheon, South Korea

    Jae-Yong Lee & Kyeong-Ho Kim

  3. Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, 156-756, South Korea

    Young-Hwan Seo & Ji Hoon Jeong

  4. Department of Drug Development Supporting Division, Seoul Pharma Laboratories Co. Ltd. (SPL), Seoul, 153-802, South Korea

    Hohyun Kim, Jae-Yong Lee, Ki-Seob Song & Young-Hwan Seo

  5. Environmental Toxico-Genomic and Proteomic Center, College of Medicine, Korea University, Anamdong 5, Sungbuk ku, Seoul, 136-705, Republic of Korea

    So-Young Park

  6. Department of Pharmacology, College of Pharmacy, Catholic University of Daegu, Gyeongbuk, 712-702, Republic of Korea

    Hyun Dong Je

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  1. Hohyun Kim
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  2. Jae-Yong Lee
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  3. Ki-Seob Song
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  4. Young-Hwan Seo
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  6. Kyeong-Ho Kim
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  7. Hyun Dong Je
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  8. Sang Beom Han
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  9. Ji Hoon Jeong
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Corresponding authors

Correspondence to Sang Beom Han or Ji Hoon Jeong.

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Kim, H., Lee, JY., Song, KS. et al. Simultaneous Separation and Determination of Isosorbide Dinitrate and Isosorbide 5-Mononitrate in Human Plasma by LC–MS–MS. Chroma 71, 595–602 (2010). https://doi.org/10.1365/s10337-010-1480-6

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  • DOI: https://doi.org/10.1365/s10337-010-1480-6

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