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Simultaneous Determination of Fluoroquinolones, Tetracyclines and Sulfonamides in Chicken Muscle by UPLC–MS–MS

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Abstract

A rapid and sensitive analytical method for the simultaneous determination of four fluoroquinolones, four tetracyclines and six sulfonamides in chicken muscle using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC–MS–MS) has been developed and validated. Samples were extracted with McIlvaine buffer-acetonitrile, defatted with n-hexane, and analyzed by UPLC–MS–MS. Solvent delay technique was applied in the analysis to remove the non-volatile phosphate and carry out farther on-line SPE clean-up. Satisfactory recoveries (55–110%) of all the veterinary drugs were demonstrated in 1, 10 and 20 μg kg−1 spiked levels with the overall RSD for intra- and inter-day of 14 analytes less than 18%. The LOD and LOQ were 0.3 and 1.0 μg kg−1, respectively. Quantitative results of 103 real samples indicated that the present method was suitable for the quantitative analysis of real samples.

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Acknowledgments

This work was supported by the National Basic Science Research for the 11th Five-Year plan (No.2006BAD31B09-2). The authors appreciate the cooperation of other faculty members in the Department of Pharmacology and Toxicology of the Veterinary Medicine at the China Agriculture University.

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Authors and Affiliations

  1. National Reference Laboratories for Veterinary Drug Residue, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China

    Jianzhong Shen, Liming Guo, Fei Xu, Xi Xia, Xiaowei Li & Shuangyang Ding

  2. Institute for Agri-Food Standards and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, 201100, People’s Republic of China

    Qinxiong Rao

Authors
  1. Jianzhong Shen
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  2. Liming Guo
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  3. Fei Xu
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  4. Qinxiong Rao
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  5. Xi Xia
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  6. Xiaowei Li
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  7. Shuangyang Ding
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Corresponding author

Correspondence to Shuangyang Ding.

Additional information

J. Shen and L. Guo contributed equally to this work.

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Shen, J., Guo, L., Xu, F. et al. Simultaneous Determination of Fluoroquinolones, Tetracyclines and Sulfonamides in Chicken Muscle by UPLC–MS–MS. Chroma 71, 383–388 (2010). https://doi.org/10.1365/s10337-009-1463-7

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