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Improved Method for the Determination of Cyanuric Acid in Animal Feed by GC–MS

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Abstract

A specific and sensitive analytical method for the quantitative determination of cyanuric acid in animal feed was developed. Sample preparation involved the diethylamine/acetonitrile/water extraction of feed using sonication and shaking. The extract was subjected to clean-up by dual solid phase extraction using mixed mode anionic and cationic extraction cartridges. After removal of clean-up solvent, cyanuric acid was converted to a tert-butyldimethylsilyl derivative and was determined by gas chromatography–mass spectrometry in the selected ion monitoring mode. 13C 153 N3-cyanuric acid was employed as the internal standard. The calibration curve was found to be linear up to 4 mg kg−1. LOD and LOQ were determined to be 0.06 to 0.4 mg kg−1 for fish and chicken feed. The mean recovery of cyanuric acid was 96 to 98% with relative pooled standard deviation of 1.8–7.4% in the range of 0.5 to 100 mg kg−1 for fish and chicken feed. The validated method was applicable for analysing cyanuric acid in animal feed.

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Acknowledgments

The authors sincerely thank Dr T.L. Ting, Government Chemist of the Government Laboratory of the HKSAR, for his continuous support and the fruitful discussion with Dr. Amos Cheng during the course of the study. The contents of this paper do not necessarily reflect the views of the Government of the HKSAR, nor does mention of trade names or commercial products constitute endorsement or recommendations of use.

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Authors and Affiliations

  1. Government Laboratory, 7/F Homantin Government Offices, 88 Chung Hau Street, Ho Man Tin, Kowloon, Hong Kong

    Hubert P. O. Tang, Shirley S. L. Lai, Ashley Y. H. Lai & W. O. Lee

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  1. Hubert P. O. Tang
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  2. Shirley S. L. Lai
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  3. Ashley Y. H. Lai
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  4. W. O. Lee
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Correspondence to Hubert P. O. Tang.

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Tang, H.P.O., Lai, S.S.L., Lai, A.Y.H. et al. Improved Method for the Determination of Cyanuric Acid in Animal Feed by GC–MS. Chroma 70, 1405–1410 (2009). https://doi.org/10.1365/s10337-009-1315-5

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  • DOI: https://doi.org/10.1365/s10337-009-1315-5

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