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Simultaneous Determination of 6β-Hydroxycortisol and 6β-Hydroxycortisone in Human Urine by LC with UV Absorbance Detection

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Abstract

A liquid chromatographic method for the simultaneous determination of 6β-hydroxycortisol and 6β-hydroxycortisone using dexamethasone as internal standard in human urine is described. Separation was achieved on a reversed-phase C18 column by a gradient elution of acetonitrile and water containing 0.1% formic acid. 6β-Hydroxycortisol and 6β-hydroxycortisone were monitored by UV absorption at 244 nm. The lower limits of quantitation were 5 ng mL−1 for both analytes. The intra-day and inter-day relative standard deviations were less than 7.4%. Accuracy determined at three concentrations ranged between 92.16 and 109.77%. The sensitivity, specificity and accuracy of this method were demonstrated to be satisfactory for measuring both metabolites in human urine.

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Acknowledgment

We thank the Health Department of Hunan Province for the support on this work.

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Authors and Affiliations

  1. Research Institute of Drug Metabolism and Pharmacokinetics, School of Pharmaceutical Sciences, Central South University, 410013, Changsha, Hunan, People’s Republic of China

    Xi Luo, Wei Wu, Xiaoxi Sheng, Ningfang Cai & Zeneng Cheng

  2. The Third Xiangya Hospital, Central South University, 410013, Changsha, Hunan, People’s Republic of China

    Lijun Zhu & Shikun Liu

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  1. Xi Luo
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  2. Lijun Zhu
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  3. Wei Wu
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  7. Zeneng Cheng
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Correspondence to Zeneng Cheng.

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Luo, X., Zhu, L., Wu, W. et al. Simultaneous Determination of 6β-Hydroxycortisol and 6β-Hydroxycortisone in Human Urine by LC with UV Absorbance Detection. Chroma 70, 1215–1219 (2009). https://doi.org/10.1365/s10337-009-1267-9

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  • DOI: https://doi.org/10.1365/s10337-009-1267-9

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