Abstract
A high-performance liquid chromatography-diode array detection method was developed and validated for the simultaneous determination of eight marker components, ginsenoside Rf, Rb1, Rb2, Rb3, Rd, Rg3, 20(S)-ginsenoside F2 and schizandrin, present in traditional Chinese medicinal Yi-Qi-Fu-Mai preparations. Samples were prepared using a solid phase extraction procedure. Chromatographic separation was carried out on a Waters Symmetry C18 column (4.6 × 250 mm, 5 μm) by stepwise gradient elution with water (0.05% phosphoric acid, v/v) and acetonitrile as the mobile phase. Good linear relationships between were observed between peak areas and concentrations with r 2 values above 0.9990 for all the analytes. Average recoveries for the eight markers ranged from 97.7 to 100.4%. This method was found to be rapid, sensitive, accurate, and could be readily applied to the quality assessment of Yi-Qi-Fu-Mai preparations.
References
WHO/EDM/TRM (2002) WHO traditional medicine strategy 2002–2005. World Health Organization, Geneva
Luo GA, Wang YM, Cao J, Yang XD (2004) World Sci Tech Modern Trad Chin Med 4:5–12
Cheng YY, Chen MJ (2003) Chem J Inf Comput Sci 43:1068–1070
Pharmacopoeia Committee of People’s Republic of China (2005) Chinese Pharmacopoeia. Chemical Industry Press, Beijing
Cicero AF, Vitale G, Savino G, Arletti R (2003) Phytother Res 17:174–178. doi:10.1002/ptr.1262
Shin YW, Bae EA, Kim DH (2006) Arch Pharm Res 29:685–690. doi:10.1007/BF02968253
Yue PY, Wong DY, Wu PK, Leung PY (2006) Biochem Pharmacol 72:437–445. doi:10.1016/j.bcp.2006.04.034
Hancke JL, Brugos RA, Ahumada F (1999) Fitoterapia 70:451–471. doi:10.1016/S0367-326X(99)00102-1
He XG, Lian LZ, Lin LZ (1997) J Chromatogr A 757:81–87. doi:10.1016/S0021-9673(96)00685-1
Wan JB, Li SP, Rong JJ, Yi KL, Wang YT (2004) Chin J Nat Med 2:215–218
Zhang X, Zhu TQ (2001) China J Chin Mater Med 26:54
Lau AJ, Seo BH, Woo SO, Koh HL (2004) J Chromatogr A 1057:141–149. doi:10.1016/j.chroma.2004.09.069
Li L, Zhang JL, Sheng YX, Guo DA, Wang Q, Guo HZ (2005) J Pharm Biomed Anal 38:45–51. doi:10.1016/j.jpba.2005.01.014
Wan JB, Lai CM, Li SP, Lee MY, Wang YT (2006) J Pharm Biomed Anal 41:274–279. doi:10.1016/j.jpba.2005.10.023
Li L, Sheng Y, Zhang J, Guo D (2005) J Chromatogr Sci 43:421–425
Wan JB, Yang FQ, Li SP, Wang YT, Cui XM (2006) J Pharm Biomed Anal 41:1596–1601. doi:10.1016/j.jpba.2006.01.058
Wan JB, Li P, Li SP, Wang YT, Dong TT-X, Tsim KW-K (2006) J Sep Sci 29:2190–2196. doi:10.1002/jssc.200600103
Kim SN, Ha YW, Shin H, Son SH, Wu SJ, Kim YS (2007) J Pharm Biomed Anal 45:164–170. doi:10.1016/j.jpba.2007.05.001
Li L, Tsao R, Dou JP, Song FR (2005) Anal Chim Acta 536:21–28. doi:10.1016/j.aca.2004.12.064
Lai CM, Li SP, Yu H, Wan JB, Kan KW, Wang YT (2006) J Pharm Biomed Anal 40:669–678. doi:10.1016/j.jpba.2005.11.003
Gu Y, Wang GJ, Sun JG, Jia YW, Xie HT, Wang W (2006) Anal Bioanal Chem 386:2043–2053. doi:10.1007/s00216-006-0857-8
Ji QC, Harkey MR, Gershwin ME, Stern JS, Hackman RM (2001) Phytochem Anal 12:320–326. doi:10.1002/pca.593
Xu MJ, Wang GJ, Xie HT, Huang Q, Wang W, Jia YW (2008) J Ethnopharmacol 115:483–488. doi:10.1016/j.jep.2007.10.016
Guan J, Lai CM, Li SP (2007) J Pharm Biomed Anal 44:996–1000. doi:10.1016/j.jpba.2007.03.032
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhou, DD., Jiang, SJ., Tong, L. et al. Quantitative Determination of Eight Major Constituents in the Traditional Chinese Medicinal Yi-Qi-Fu-Mai Preparation by LC. Chroma 70, 969–974 (2009). https://doi.org/10.1365/s10337-009-1252-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-009-1252-3