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Simultaneous Determination of Tranexamic Acid and Losartan Potassium in Dosage Formulations and Human Serum by RP-LC

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Abstract

Rapid liquid chromatographic procedure for analytical quality control of pharmaceutical preparations and human serum containing drugs, tranexamic acid together with losartan potassium are proposed, using acetonitrile: water (50:50), adjusting pH to 2.6 with phosphoric acid as a mobile phase, UV detection at 205 nm and propylparaben sodium was used as internal standard. The results obtained showed a good agreement with the declared contents. The method shows good linearity in the range of 40–10,000 ng mL−1 for tranexamic acid serum concentrations with a correlation coefficient 0.9999 (inter- and intra-day CV <3.18) and in the range 5–10,000 ng mL−1 for losartan potassium serum concentrations with a correlation coefficient 0.9999 (inter- and intra-day CV <3.61). The recovery was >97.8%. The proposed method may be used for the quantitative analysis of tranexamic acid and losartan potassium alone or in combination from raw materials, in bulk drugs, dosage formulations and in serum.

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References

  1. Martindale, The extra pharmacopoeia (1996) Reynolds JEF (ed) Royal Pharmaceutical Society, 31st edn. pp 771, 900

  2. British Pharmacopoeia (2003) The Stationary office under license from the controller of Her Majesty’s stationary office for the department of health on behalf of the health Ministers, pp 1870–1871

  3. Arayne MS, Sultana N, Siddiqui FA, Mirza AZ, Zuberi MH (2008) J Mol Str 891(1–3):475–480. doi:10.1016/j.molstruc.2008.04.026

    Article  CAS  Google Scholar 

  4. The Merck index (1999) Whitehouse Station, 12th edn. Merck and Co, New Jersey

    Google Scholar 

  5. Arayne MS, Sultana N, Haroon U, Qureshi F, Ali SA (2006) Pak J Pharm Sci 19(2):134–141

    CAS  Google Scholar 

  6. Arayne MS, Sultana N, Qureshi F, Zuberi MH (2008) J Chem Soc Pak, conference issue

  7. Lip G, Blann A, Jones A, Lip P, Beevers D (1997) Am J Cardiol 80:1566. doi:10.1016/S0002-9149(97)00749-2

    Article  CAS  Google Scholar 

  8. Eliasson M, Jansson J, Nilsson P, Asplund K (1997) J Hypertens 15:349. doi:10.1097/00004872-199715040-00005

    Article  CAS  Google Scholar 

  9. Poli KA, Tofler GH, Larson MG, Evans JC, Sutherland PA, Lipinska I, Mittleman MA, Muller JE, D’Agostino RB, Wilson PWF, Levy D (2000) Circulation 101:264

    CAS  Google Scholar 

  10. Emiri T, Yoichi K, Yasuyuki S, Tsuneo K, Lo H (2001) Stroke 32:1336

    Google Scholar 

  11. Dielis AW, Smid M, Spronk HM, Hamulyak K, Kroon AA, Cate H (2005) Hypertension 46(6):1236–1242. doi:10.1161/01.HYP.0000193538.20705.23

    Article  CAS  Google Scholar 

  12. Matsubayashi K, Kojima C, Tachizawa H (1988) J Chromatogr A 9(433):225–234

    Google Scholar 

  13. Elworthy PM, Tsementzis SA, Westhead D, Hitchcock ER (1985) J Chromatogr 343(1):109–117

    CAS  Google Scholar 

  14. Chang Q, Yin OQ, Chow MS (2004) J Chromatogr B Analyst Technol Biomed Life Sci 805(2):275–280

    Article  CAS  Google Scholar 

  15. Ritter MA, Furtek CI, Lo MW (1997) J Pharm Biomed Anal 15(7):1021–1029. doi:10.1016/S0731-7085(96)01948-6

    Article  CAS  Google Scholar 

  16. Farthing D, Sica D, Fakhry I, Pedro A, Gehr TW (1997) J Chromatogr B Biomed Sci Appl 704(1–2):374–378

    Article  CAS  Google Scholar 

  17. Shah SA, Rathod IS, Suhagia BN, Savale SS, Patel JB (2001) J AOAC Int 84(6):1715–1723

    CAS  Google Scholar 

  18. Gonzalez L, Lopez JA, Alonso RM, Jimenez RM (2002) J Chromatogr A 949(1–2):49–60

    Article  CAS  Google Scholar 

  19. Erk N (2001) J Pharm Biomed Anal 24(4):603–611. doi:10.1016/S0731-7085(00)00434-9

    Article  CAS  Google Scholar 

  20. Carlucci G, Palumbo G, Mazzeo P, Quaglia MG (2000) J Pharm Biomed Anal 23(1):185–189

    Article  CAS  Google Scholar 

  21. International Conference on the Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use (ICH) (2006) Validation of Analytical Procedures, Methodology

  22. United States Pharmacopeia 30 (NF 25); Validation of Compendial Methods, United States Pharmacopeia Convention: Rockville

Download references

Acknowledgment

The authors would like to acknowledge the financial support provided by the Higher Education Commission, Pakistan, for this research work. Authors would also like to express gratitude to all the colleagues of Lab 9 for their generous help in small details which helped in improving overall manuscript.

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Authors and Affiliations

  1. Department of Chemistry, University of Karachi, Karachi, 75270, Pakistan

    M. Saeed Arayne, Faiza Qureshi, Farhan Ahmed Siddiqui, Agha Zeeshan Mirza, Saima Sher Bahadur & Muhammad Hashim Zuberi

  2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Karachi, 75270, Pakistan

    Najma Sultana

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  1. M. Saeed Arayne
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  2. Najma Sultana
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  3. Faiza Qureshi
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  4. Farhan Ahmed Siddiqui
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  5. Agha Zeeshan Mirza
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  6. Saima Sher Bahadur
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  7. Muhammad Hashim Zuberi
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Correspondence to M. Saeed Arayne.

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Arayne, M.S., Sultana, N., Qureshi, F. et al. Simultaneous Determination of Tranexamic Acid and Losartan Potassium in Dosage Formulations and Human Serum by RP-LC. Chroma 70, 789–795 (2009). https://doi.org/10.1365/s10337-009-1225-6

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