Skip to main content
SpringerLink
Log in

Sensitive, Selective, Precise and Accurate LC–MS Method for Determination of Clonidine in Human Plasma

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A selective and sensitive liquid chromatography–mass spectrometric method in ESI (+ve) mode was developed and validated completely in human plasma. Clonidine and IS, carbamazapine, were extracted from human plasma via liquid–liquid extraction with ethyl acetate. Following evaporation under nitrogen, the residue was reconstituted with mobile phase and analyzed using API 4000 LC–MS–MS system. An isocratic program with binary mobile phases (0.1% formic acid in water and acetonitrile) was used to separate interference peaks by a C18 analytical column. Linearity range was 0.49–73.98 ng mL−1. The intra- and inter-day accuracy and precision were within acceptable limits (≤15%). This method was successfully applied to a single dose 25 μg tablets BE study of clonidine in healthy male volunteers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. National Institute of Neurological Disorders and Stroke (2002) http://www.ninds.nih.gov/news_and_events/news_articles/news_article_adhd.htm

  2. Schapiro NA (2002) Dude, you don’t have Tourette’s: Tourette’s syndrome, beyond the tics. Pediatr Nurs. 28: 243–246, 249–253. PMID 12087644

    Google Scholar 

  3. Howard S (2008) Illustrated pharmacology memory cards: pharmnemonics. Minireview, 12. ISBN 1-59541-101-1

  4. Gourlay SG, Stead LF, Benowitz NL (2004) Clonidine for smoking cessation. Cochrane Database Syst Rev 2004, (2) Art. No.: CD000058. doi:10.1002/14651858.CD000058.pub2

  5. Müller C, Ramic M, Harlfinger S, Hünseler C, Theisohn M, Roth B (2007) J Chromatogr A 1139(2). doi:10.1016/j.chroma.2006.11.020

  6. Gergov M, Ojanperä I, Vuori E (2003) J Chromatogr B 795(1). doi:10.1016/S1570-0232(03)00498-7

  7. Pelzer M, Addison T, Li W, Jiang X, Weng N (2002) J Liq Chromatogr Relat Technol 25(7). doi:10.1081/JLC-120003421

  8. Parekh SA, Pudage A, Joshi SS, Vaidya VV, Gomes NA (2008) J Chromatogr B 867 (2). doi:10.1016/j.jchromb.2008.03.030

  9. Ke GM, Wang L, Xue HY, Lu WL, Zhang X, Zhang Q, Guo HY (2005) Biol Pharm Bull 28

  10. Wenzl T, Lankmayr EP, Wintersteiger R, Sadjak A, Likar R, Zakel D (2002) J Biochem Biophys Methods 53(1–3). doi:10.1016/S0165-022X(02)00101-X

  11. Guidance for Industry, Bioanalytical Method Validation (2001) Food and Drug Administration, Center for Drug Evaluation and Research (CDER)

  12. Shah VP, Midha KK, Findlay JWA, Hill HM, Hulse JD, McGilvery IJ, McKay G, Miller KJ, Patnaik RN, Powel ML, Tonelli A, Viswanathan CT, Yacobi A (2000) Pharm Res 17:1551. doi:10.1023/A:1007669411738

    Article  CAS  Google Scholar 

  13. McDoall RD, Hartman C, Penninkx W, Hayden YV, Pankeerberghen V, Massart DL (1995) In: Blume HH, Midha KK bio’94, bio-international 2, bioavailability, bioequivalence and pharmacokinetic studies, Medpharm Scientific, Stuttguart, Germany, p 331

  14. Nagaraja NV, Paliwal JK, Gupta RC (1999) J Pharm Biomed Anal 20:433. doi:10.1016/S0731-7085(98)00256-8

    Article  CAS  Google Scholar 

  15. Almeida AM, CastelBranco MM, Falcao AC (2002) J Chromatogr B 774:215. doi:10.1016/S1570-0232(02)00244-1

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cadila Pharmaceuticals Limited, 1389-Trasad Road, Dholka, Gujarat, India

    Chinmoy Ghosh, Rajendra P. Singh, Shafi Inamdar, Mandar Mote & Bhaswat S. Chakraborty

Authors
  1. Chinmoy Ghosh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rajendra P. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shafi Inamdar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mandar Mote
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bhaswat S. Chakraborty
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chinmoy Ghosh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ghosh, C., Singh, R.P., Inamdar, S. et al. Sensitive, Selective, Precise and Accurate LC–MS Method for Determination of Clonidine in Human Plasma. Chroma 69, 1227–1232 (2009). https://doi.org/10.1365/s10337-009-1102-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-009-1102-3

Keywords

Search

Navigation