Skip to main content
SpringerLink
Log in

Simultaneous Determination of Twelve Saponins in Radix et Rhizoma Notoginseng by Rapid Resolution LC-ESI-TOF-MS

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Rapid resolution liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry was developed for simultaneous determination of twelve saponins in Radix et Rhizoma Notoginseng (RRN) with macranthoidine A as internal standard. All calibration curves showed good linear regression (r 2 > 0.9935) within test range. The limits of detection of the twelve compounds were in the range of 1.53–5.08 ng mL−1. This method was successfully applied to analyze the saponins in fifteen samples of RRN.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. National Commissionof Chinese Pharmacopoeia (2005) Pharmacopoeia of People’s Republic of China. Chemical Industry Press, Beijing, pp 10–11

    Google Scholar 

  2. Chan P, Thomas GN, Tomlinson B (2002) Acta Pharmacol Sin 23:1157–1162

    CAS  Google Scholar 

  3. Sun K, Wang CS, Guo J, Horie Y, Fang SP, Wang F et al (2007) Life Sci 81:509–518. doi:10.1016/j.lfs.2007.06.008

    Article  CAS  Google Scholar 

  4. Liu L, Shi R, Shi Q, Cheng Y, Huo Y (2008) Planta Med 74:203–209. doi:10.1055/s-2008-1034303

    Article  CAS  Google Scholar 

  5. Rhule A, Navarro S, Smith JR, Shepherd DM (2006) J Ethnopharmacol 106:121–128. doi:10.1016/j.jep.2005.12.012

    Article  Google Scholar 

  6. Chen FD, Wu MC, Wang HE, Hwang JJ, Hong CY, Huang YT et al (2001) Am J Chin Med 29:517–524. doi:10.1142/S0192415X0100054X

    Article  CAS  Google Scholar 

  7. Lin CF, Wong KL, Wu RSRSC, Huang TC, Liu CF (2003) Phytother Res 17:1119–1122. doi:10.1002/ptr.1329

    Article  Google Scholar 

  8. Sun HX, Ye YP, Pan HJ, Pan YJ (2004) Vaccine 22:3882–3889. doi:10.1016/j.vaccine.2004.04.012

    Article  CAS  Google Scholar 

  9. Chen SW, Li XH, Ye KH, Jiang ZF, Ren XD (2004) Acta Pharmacol Sin 25:1151–1156

    Google Scholar 

  10. Wang CZ, McEntee E, Wicks S, Wu JA, Yuan CS (2006) J Nat Med 60:97–106. doi:10.1007/s11418-005-0027-x

    Article  CAS  Google Scholar 

  11. Yang ZG, Chen A, Sun HX, Ye YP, Fang WH (2007) Vaccine 25:161–169. doi:10.1016/j.vaccine.2006.05.075

    Article  CAS  Google Scholar 

  12. Du QZ, Jerz G, Waibel R, Winterhalter P (2003) J Chromatogr A 1008:173–180. doi:10.1016/S0021-9673(03)00988-9

    Article  CAS  Google Scholar 

  13. Vanhaelen-Fastre RJ, Faes ML, Vanhaelen MH (2000) J Chromatogr A 868:269–276. doi:10.1016/S0021-9673(99)01253-4

    Article  CAS  Google Scholar 

  14. Cui JF, Bjorkhem I, Eneroth P (1997) J Chromatogr B Analyt Technol Biomed Life Sci 689:349–355. doi:10.1016/S0378-4347(96)00304-0

    Article  CAS  Google Scholar 

  15. Gao XF, Dan M, Zhao AH, Xie GX, Jia W (2008) Biomed Chromatogr 22:244–249. doi:10.1002/bmc.915

    Article  CAS  Google Scholar 

  16. Wei YJ, Qi LW, Li P, Luo HW, Yi L, Sheng LH (2007) J Pharm Biomed Anal 45:775–784. doi:10.1016/j.jpba.2007.07.013

    Article  CAS  Google Scholar 

  17. Zhang HJ, Cheng YY (2006) J Pharm Biomed Anal 40:429–432. doi:10.1016/j.jpba.2005.07.010

    Article  CAS  Google Scholar 

  18. Li L, Tsao R, Dou JP, Song FR, Liu ZQ, Liu SY (2005) Anal Chim Acta 536:21–28. doi:10.1016/j.aca.2004.12.064

    Article  CAS  Google Scholar 

  19. Castro-Perez J, Plumb R, Granger JH, Beattie I, Joncour K, Wright A (2005) Rapid Commun Mass Spectrom 19:843. doi:10.1002/rcm.1859

    Article  CAS  Google Scholar 

  20. Plumb RS, Granger JH, Stumpf CL, Johnson KA, Smith BW, Gaulitz S et al (2005) Analyst (Lond) 130:844. doi:10.1039/b501767j

    Article  CAS  Google Scholar 

  21. Guan J, Lai CM, Li SP (2007) J Pharm Biomed Anal 44:996–1000. doi:10.1016/j.jpba.2007.03.032

    Article  CAS  Google Scholar 

  22. Chan ECY, Yap SL, Lau AJ, Leow PC, Toh DF, Koh HL (2007) Rapid Commun Mass Spectrom 21:519–528. doi:10.1002/rcm.2864

    Article  CAS  Google Scholar 

  23. Wei GM, Masanori M, Karl EM, Shao LL, Bertrand D, Satoshi T (1999) Phytochemistry 52:1133–1139. doi:10.1016/S0031-9422(99)00364-7

    Article  Google Scholar 

  24. Mao Q, Cao D, Jia XS (1993) Acta Pharmacol Sin 28:273–281

    CAS  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the key program (No. 30530870) of the National Science Foundation of China.

Author information

Authors and Affiliations

  1. Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education, 210009, Nanjing, China

    Hai-Ling Liu, Li Xia, Jun Cao, Ping Li & Lian-Wen Qi

Authors
  1. Hai-Ling Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jun Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ping Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lian-Wen Qi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ping Li.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, HL., Xia, L., Cao, J. et al. Simultaneous Determination of Twelve Saponins in Radix et Rhizoma Notoginseng by Rapid Resolution LC-ESI-TOF-MS. Chroma 68, 1033–1038 (2008). https://doi.org/10.1365/s10337-008-0796-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0796-y

Keywords

Search

Navigation