Skip to main content
SpringerLink
Log in

Validated, Stability-Indicating LC Method for Analysis of Pipenzolate Bromide and Its Hydrolysis Products

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A liquid chromatographic method has been developed and validated for quantitative analysis of pipenzolate bromide (PP), its hydrolysis products, and phenobarbitone, sodium benzoate, and sodium saccharine. A 5-μm particle ODS column was used with acetonitrile–KH2PO4 (10 mm, pH 3.5) 40:60 (v/v), containing 5 mm heptanesulfonic acid sodium salt, as mobile phase. Quantitation was achieved by UV detection at 210 nm, on the basis of peak area. Forced degradation studies were performed on a bulk sample of PP using 0.1 M hydrochloric acid, 0.01 M sodium hydroxide, 0.33% hydrogen peroxide, heat (70 °C), and photolytic degradation. The proposed LC method was used to study the kinetics of acidic hydrolysis and pH-rate profiles of hydrolysis of PP in Britton–Robinson buffer solutions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Sweetman SC (ed) (2002) Martindale—the complete drug reference, 33rd edn. Pharmaceutical Press, London

  2. Budavari S, Smith A, Kinneary JF (eds) (1996) The Merck index, 12th edn. Merck Research Laboratories

  3. Stanley SMR, Foo HC (2006) J Chromatogr B Analyt Technol Biomed Life Sci 836:1–14. doi:10.1016/j.jchromb.2006.03.034

    Article  CAS  Google Scholar 

  4. Yiu KCH, Ho ENM, Wan TSM (2004) Chromatographia 59:S45–S50. doi:10.1365/s10337-004-0233-9

    Article  CAS  Google Scholar 

  5. Tang FPW, Leung GNW, Wan TSM (2001) Electrophoresis 22:2201–2209. doi:10.1002/1522-2683(20017)22:11<2201::AID-ELPS2201>3.0.CO;2-S

    Google Scholar 

  6. Ozkan SA, Erk N, Senturk Z (1999) Anal Lett 32:497–520. doi:10.1080/00032719908542836

    Article  CAS  Google Scholar 

  7. Sabnis DS, Shirodkar MM (1985) Indian Drugs 23:50–51

    CAS  Google Scholar 

  8. International conference on harmonization (1993) Stability testing of new drug substances and products. ICH, Geneva

  9. Snyder LR, Kirkland JJ, Glajch JL (1997) Practical HPLC method development, 2nd edn. Wiley, New York, p 317

    Google Scholar 

  10. European Agency for the Evaluation of Medical Products (1996) ICH Topic Q 2B Note for guidance on validation of analytical procedures: methodology CPMP/ICH/281/95

  11. British Pharmacopoeia (2003) The Stationery Office, London

  12. The United States Pharmacopeia 30 (2007) The National Formulary 25, United States Pharmacopeial Convention, INC. p 249–253

Download references

Author information

Authors and Affiliations

  1. Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Suez Canal University, Ismailia, 41522, Egypt

    Ghada M. Hadad

Authors
  1. Ghada M. Hadad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ghada M. Hadad.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hadad, G.M. Validated, Stability-Indicating LC Method for Analysis of Pipenzolate Bromide and Its Hydrolysis Products. Chroma 68, 207–212 (2008). https://doi.org/10.1365/s10337-008-0715-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0715-2

Keywords

Search

Navigation