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Development and Validation of LC-UV for Simultaneous Estimation of Rosiglitazone and Glimepride in Human Plasma

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Abstract

A simple, sensitive and specific liquid chromatographic method with UV detection (228 nm) was developed for the simultaneous estimation of rosiglitazone and glimepride in human plasma. Rosiglitazone and glimepride were extracted from plasma using liquid–liquid extraction. Separation was achieved with an RP C18 Column using a mixture of phosphate buffer (50 mM) with octane sulfonic acid (10 mM), methanol and acetonitrile as a mobile phase (55:10:35, v/v). pH was adjusted to 7.0. Amlodipine was used as an internal standard (IS). LOD of the method was found to be 20 ng mL−1 for both drugs. Results were linear over the studied range 40.994–2007.556 ng mL−1 for rosiglitazone (r 2 ≥ 0.99) and 41.066–2094.84 ng mL−1 for glimepride( r 2 ≥ 0.99). The method was found to be simple, selective, precise and reproducible for the estimation of both drugs from spiked human plasma.

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References

  1. Patel J, Miller E, Patwardhan R (1998) Diabetes 47(Suppl 1):A17

    Google Scholar 

  2. Young PW, Buckle DR, Cantello BC, Chapman H, Clapham JC, Coyle PJ, Haigh D, Hindley RM, Holder JC, Kallender H, Latter AJ, Lawrie KWM, Massakowska D, Murphy GJ, Roxbee Cox L, Smith SA (1998) J Pharmacol Exp Ther 284:7

    Google Scholar 

  3. Malerczyk V, Badian M, Korn A, Lehr KH, Waldhansl W (1994) Drug Metab Drug Interact 11:341

    CAS  Google Scholar 

  4. Ledercq-Meyer V, Akkan AG, Marchand J, Malaisse WJ (1991) Biochem Pharmacol 42:1634

    Article  CAS  Google Scholar 

  5. Ho EN , Yiu KC, Wan TS, Stewart BD, Watkins KL (2004) J Chromatogr., B: Anal Technol Biomed Life Sci 811:65–73

    Google Scholar 

  6. Radhakrishna T, Satyanarayana J, Satyanarayana A (2002) J Pharm Biomed Anal 29:873–880

    Article  CAS  Google Scholar 

  7. Muxlow AM, Fowles S, Russell P (2001) J Chromatogr B 752:77–84

    Article  CAS  Google Scholar 

  8. Kolte BL, Raut BB, Deo AA, Bagool MA Shinde DB (2003) J Chromatogr B 788:37–44

    Article  CAS  Google Scholar 

  9. Kim KA, Park JY (2004) Biomed Chromatogr:613–615

  10. Gomes P, Sippel J, Jablonski A, Steppe M (2004) J Pharm Biomed Anal 36:909–913

    Article  CAS  Google Scholar 

  11. Rao NVS, Biju B, Mullangi R (2003) Biomed Chromatogr 17:417–420

    Article  Google Scholar 

  12. Yun-Kyoung S, Jeong-Eun M, Chong-Kook K (2004) J Chromatogr B 810:143–149

    Google Scholar 

  13. Petra K, Klimes J, Dohnal J, Tisovska L (2004) J Pharm Biomed Anal 36:205–209

    Article  Google Scholar 

  14. Khan MA, Sinha S, Vartak S, Bhartiya A, Kumar SJ (2005) Pharm Biomed Anal: 928–943

  15. Lehr KH, Damm P (1990) J Charmatogr 526:497

    Article  CAS  Google Scholar 

  16. Khabbaz RL, Daud AR, Zoghbi A (2005) J Liq Chromatogr Relat Technol 28:3255–3263

    Article  Google Scholar 

  17. Salem II, Idrees J, AI Tamimi JI (2004) J Chromatogr B 799:103

    Article  CAS  Google Scholar 

  18. Kolte BL, Raut BB, Deo AA, Bagool MA, Shinde DB (2004) J Chromatogr Sci:70–73

  19. Vankatesh P, Harisudhan T, Srinivas NR (2006) Biomed Chromatogr:1043–1048

  20. El Deeb S, Schepers U, Watzig H (2006) J Sep Sci:1571–1577

  21. Sane RT, Menon SN, Gunesh G (2004) Chromatographia:451–453

  22. Bhaskar KL, Bharat BR, Adwait AD, Manoj AB, Devanand BS (2005) J Sep Sci 2076–2079

  23. Guidance for Industry-Bioanalytical Method Validation (2001) US Department Of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Veterinary Medicine

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Authors and Affiliations

  1. Pharmaceutical Quality Assurance Division of Pharmacy, Faculty of Technology and Engineering, M. S. University, Baroda, 390 001, India

    Jahanvi N. Jingar & Sadhna J. Rajput

  2. Synchron Research Services Pvt. Ltd, Sarkhej Gandhinagar Highway, Bodakdev, Ahmedabad, 380 054, India

    Bhavesh Dasandi & Shivprakash Rathnam

Authors
  1. Jahanvi N. Jingar
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  2. Sadhna J. Rajput
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  3. Bhavesh Dasandi
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  4. Shivprakash Rathnam
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Correspondence to Jahanvi N. Jingar.

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Jingar, J.N., Rajput, S.J., Dasandi, B. et al. Development and Validation of LC-UV for Simultaneous Estimation of Rosiglitazone and Glimepride in Human Plasma. Chroma 67, 951–955 (2008). https://doi.org/10.1365/s10337-008-0633-3

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  • DOI: https://doi.org/10.1365/s10337-008-0633-3

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