Skip to main content
SpringerLink
Log in

Separation and Identification of Ergosta-4,6,8(14),22-tetraen-3-one from Ganoderma atrum by High-Speed Counter-Current Chromatography and Spectroscopic Methods

  • Limited Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

High speed counter-current chromatography in semi-preparative scale was used to separate and purify ergosta-4,6,8(14),22-tetraen-3-one from Ganoderma atrum, a famous traditional Chinese medicine. A two-phase solvent system composed of a mixture of n-hexane–ethanol–water (6: 5: 1, v/v/v) was used and the separation conditions were optimized. In a typical run in less than 400 min, 100 mg of samples can be separated to yield 14 mg of ergosta-4,6,8(14),22-tetraen-3-one with 99.1% purity. The structure of this compound was elucidated by UV, EI-MS, 1H NMR and 13C NMR.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Lin ZB (2001) Modern research of Lingzhi: Lingzhi species in China, 2nd edn. Beijing Medical University Press, Beijing

    Google Scholar 

  2. Kohda H, Tokumoto W, Sakamoto K, Fujii M, Hirai Y, Ymasaki K, Komoda Y, Nakamura H, Ishihara S, Uchida M (1985) Chem Pharm Bull 33:1367–1374

    CAS  Google Scholar 

  3. Zeng XL, Bao HY (2004) J Fungal Res 12:68–77

    Google Scholar 

  4. Hirotani M, Furuya T, Shiro M (1985) Phytochemistry 24:2055–2061

    Article  CAS  Google Scholar 

  5. Nishitoba T, Sato H, Sakamura S (1987) Agric Biol Chem 51:1149–1153

    CAS  Google Scholar 

  6. Wang XM, Yang M, Guan SH, Liu RX, Xia JM, Bi KS, Guo DA (2006) J Pharm Biomed Anal 41:838–844

    Article  CAS  Google Scholar 

  7. Ma CH, Ke W, Sun ZL, Peng JY, Li ZX, Zhou X, Fan GR, Huang CG (2006) Chromatographia 64:83–87

    Article  CAS  Google Scholar 

  8. Chen JH, Wang FM, Lee FSC, Wang XR, Xie MY (2006) Talanta 69:172–179

    Article  CAS  Google Scholar 

  9. Ito Y (2005) J Chromatogr A 1065:145–168

    Article  CAS  Google Scholar 

  10. Liu HB, Cui CB, Gu QQ, Cai B, Zhao QC, Guan HS (2005) Chin Pharm J 40:414–416

    CAS  Google Scholar 

  11. Chobot V, Opletal L, Jahodar L, Patel AV, Dacke CG, Blunden G (1997) Phytochemistry 45:1669–1671

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The financial support for this study by Program for Changjiang Scholars and Innovative Research Team in University (No: IRT0540), and by Jiangxi Provincial Department of Science and Technology is gratefully acknowledged.

Author information

Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Nanchang University, 330047, Nanchang, China

    Mingyue Shen, Mingyong Xie, Shaoping Nie, Yuanxing Wang, Junhui Chen, Chang Li & Jing Li

Authors
  1. Mingyue Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mingyong Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaoping Nie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuanxing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Junhui Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mingyong Xie.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shen, M., Xie, M., Nie, S. et al. Separation and Identification of Ergosta-4,6,8(14),22-tetraen-3-one from Ganoderma atrum by High-Speed Counter-Current Chromatography and Spectroscopic Methods. Chroma 67, 999–1001 (2008). https://doi.org/10.1365/s10337-008-0616-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0616-4

Keywords

Search

Navigation