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Simultaneous Determination of Baicalin, Baicalein, Wogonin, Oxysophocarpine, Oxymatrine and Matrine in the Chinese Herbal Preparation of Sanwu-Huangqin-Tang by Ion-Paired HPLC

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Abstract

A sensitive and reliable ion-paired high-performance liquid chromatographic method has been established for the simultaneous quantification of six major active ingredients, namely baicalin, baicalein, wogonin, oxysophocarpine, oxymatrine and matrine in the Chinese herbal preparation, Sanwu-Huangqin-Tang. HPLC analyses were performed on a Phenomenex luna C18 column with mobile phase of methanol–acetonitrile–aqueous phosphoric acid at a flow rate of 0.9 mL min−1. The complete separation was achieved within 35 min for the six target constituents. A good linear regression relationship between peak-areas and concentrations was obtained over the range of 12.10–242.0 μg*mL−1 for baicalin, 5.05–101.0 μg*mL−1 for baicalein, 0.95–19.0 μg*mL−1 for wogonin, 2.75–55.0 μg*mL−1 for oxysophocarpin, 2.75–55.0 μg*mL−1 for oxymatrine and 4.90–98.0 μg*mL−1 for matrine, respectively. The repeatability was evaluated by intra- and inter-day assays with relative standard deviation (RSD) being less than 5.1%. The recoveries, measured at three concentration levels, varied from 93.8 to 102.1%. The assay was successfully applied for determination of six bioactive compounds in Sanwu-Huangqin-Tang. The interaction of chemical constituents was observed when the herbs were used in compatibility. The results indicated that the developed assay method was rapid, accurate and could be readily utilized as a quality control method for Sanwu-Huangqin-Tang.

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, Liaoning Province, People’s Republic of China

    Lei Zhang, Liang Xu, Xiao-Jie Tan, Wei Guo, Xiao-Hui Chen & Kai-Shun Bi

  2. College of Chinese Traditional Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510006, Guangdong Province, People’s Republic of China

    Lei Zhang & Qiong-Feng Liao

  3. Analysis and Testing Center, Inner Mongolia University for Nationalities, 42 Huolinhe Road, Tongliao, 028042, Inner Mongolia, People’s Republic of China

    Liang Xu

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  1. Lei Zhang
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  2. Liang Xu
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  3. Xiao-Jie Tan
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  4. Qiong-Feng Liao
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  5. Wei Guo
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  7. Kai-Shun Bi
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Correspondence to Kai-Shun Bi.

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Zhang, L., Xu, L., Tan, XJ. et al. Simultaneous Determination of Baicalin, Baicalein, Wogonin, Oxysophocarpine, Oxymatrine and Matrine in the Chinese Herbal Preparation of Sanwu-Huangqin-Tang by Ion-Paired HPLC. Chroma 66, 115–120 (2007). https://doi.org/10.1365/s10337-007-0260-4

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  • DOI: https://doi.org/10.1365/s10337-007-0260-4

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