Abstract
The dry rhizome of Acorus tatarinowii Schott is a traditional Chinese medicine (TCM) which has been used to treat many diseases, for example epilepsy, for thousands of years. In this work, microwave distillation and simultaneous solid-phase microextraction (MD-SPME) were used for analysis of the essential oil in the fresh leaves of Acorus tatarinowii. Isolation, extraction, and concentration of the volatile constituents of the leaves can be completed rapidly, in a single step, by use of MD-SPME; the compounds can then be analyzed by gas chromatography-mass spectrometry (GC-MS). MD-SPME conditions, including microwave power, irradiation time, and SPME fiber coating, were studied. By use of MD-SPME-GC-MS twenty-nine compounds were identified, for the first time, in the essential oil of the plant leaves; the compounds were the same as those in the rhizomes. This suggests the leaves of the plant might be used as a TCM. To demonstrate the feasibility of the method, conventional steam distillation (SD) was also used for extraction of the essential oil from the leaves. The same compounds, in similar amounts, were identified by both methods, confirming the MD-SPME method is highly reliable. Compared with SD, MD-SPME required less time (only 2 min), less sample (1.0 g), and no organic solvent. These results are indicative of the suitability of MD-SPME-GC-MS for simple, rapid, and solvent-free analysis of plant essential oils.
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References
Wen KC, Huang CY, Lu FL (1993) J Chromatogr 631:241–248
Tang HM (2002) Lishizhen Med Mater Res 13:1–4
Chen L, Liao WP, Lu XF, Sun WW, Gao MM (2003) Epilepsia 44:177–177
Deng CH, Li N, Zhang XM (2004) J Chromatogr A 1059:149–155
Hao Q, Ha CY (2000) Chin J Anal Chem 28:300–302
Guo FQ, Liang YZ, Xu CJ, Li XN, Huang LF (2004) J Pharm Biomed Anal 35:469–478
Li HJ, Meng XY, Wu Y, Bao YL, Li YX (2004) Chem Res Chin U 20:311–313
Yu JQ, Lei JC, Yu HD, Cai X, Zou GL (2004) Phytochem 65:881–884
Huang LF, Li BY, Liang YZ, Guo FQ, Wang YL (2004) Anal Bioanal Chem 378:510–517
Guo FQ, Liang YZ, Xu CJ, Huang LF (2003) J Chromatogr A 1016:99–110
Li R, Jiang ZT (2004) Flavour Fragr J 19:311–313
Zhu SY, Yang Y, Yu HD, Yue Y, Zou GL (2005) J Ethnopharmacol 96:151–158
Chen SS, Spird M (1994) J Microwave Power Electromagn Energy 29:231–236
Bilia AR, Flamini G, Taglioli V, Morelli I, Vincieri FF (2002) Food Chem 76:307–310
Stashenko EE, Cervantes M, Combariza Y, Fuentes H, Martinez JR (1999) J High Res Chromatogr 22:343–348
Jain MP, Sama JK, Jain SM, Sharma VK, Singh B (2003) Indian J Chem Tech 10:370–374
Silva MGD, Dos Santos RN, Matos FJA, Machado MIL (2003) Flavour Fragr J 18:303–304
Silva MGD, Matos FJA, Machado MIL, Silva FDO (2004) J Essent Oil Res 16:189–194
Lo Presti M, Ragusa S, Trozzi A, Dugo P, Visinoni F, Fazio A, Dugo G, Mondello L (2005) J Sep Sci 28:273–280
Stashenko EE, Jaramillo BE, Martinez JR (2004) J Chromatogr A 1025:93–103
Stashenko EE, Jaramillo BE, Martinez JR (2004) J Chromatogr A 1025:105–113
Lucchesi ME, Chemat F, Smadja J (2004) J Chromatogr A 1043:323–327
Deng CH, Xu XQ, Zhang XM (2006) Anal Chim Acta 556:289–294
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Revised: 24 February and 20 March 2006
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Ye, H., Ji, J., Deng, C. et al. Rapid Analysis of the Essential Oil of Acorus tatarinowii Schott by Microwave Distillation, SPME, and GC-MS. Chroma 63, 591–594 (2006). https://doi.org/10.1365/s10337-006-0796-8
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DOI: https://doi.org/10.1365/s10337-006-0796-8