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Simultaneous RP-LC Determination of Additives in Soft Drinks

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Abstract

A reverse-phase HPLC method for the simultaneous determination of the main artificial sweeteners, preservatives and dyes present in soft drinks is proposed. It involves the use of a 10 μm LiChrosorb RP18 column and a binary eluent consisting of aqueous 0.1 M phosphate buffer (pH 4.0) added with methanol, according to a suitable gradient elution program. Good separations were obtained within less than 20-min run-time, with a satisfactory precision. The sensitivity of spectrophotometric detection was optimised by adopting a wavelength switching technique, thus achieving for all the additives considered detection limits ranging from 0.1 to 3.0 mg L−1, well below the maximum permitted levels. The method was applied to some commercial soft drinks, whose analysis required minimum pre-treatment before direct injection.

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Authors and Affiliations

  1. Department of Chemical Sciences and Technology, University of Udine, via Cotonificio 108, 33100, Udine, Italy

    N. Dossi, R. Toniolo, S. Susmel, A. Pizzariello & G. Bontempelli

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  1. N. Dossi
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  2. R. Toniolo
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  3. S. Susmel
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  4. A. Pizzariello
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  5. G. Bontempelli
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Correspondence to G. Bontempelli.

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Revised: 7 November 2005 and 30 March 2006

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Dossi, N., Toniolo, R., Susmel, S. et al. Simultaneous RP-LC Determination of Additives in Soft Drinks. Chroma 63, 557–562 (2006). https://doi.org/10.1365/s10337-006-0793-y

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  • DOI: https://doi.org/10.1365/s10337-006-0793-y

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