Abstract
Cortex Moutan is one important traditional Chinese herb used in many traditional prescriptions. Paeonol (PN) and paeoniflorin (PF) are two active components contained in Cortex Moutan. In the present work, micellar electrokinetic capillary chromatography (MEKC) was applied to the determination of these active components in Cortex Moutan, and the analytical results were compared with that obtained by high-performance liquid chromatography (HPLC). The optimized buffer system containing 10 mM borate and 25 mM SDS at pH 9.5 was employed in MEKC analysis and the linearities between peak areas and the concentrations of the analytes were investigated. The excellent linear behavior was exhibited over the investigated concentration ranges (R2: 0.9945 for PN and 0.9992 for PF in MEKC, while 0.9993 for PN and 0.9998 for PF in HPLC). The recoveries of the developed MEKC method ranged 97.1–104.4% for PN and 95.3–103.2% for PF, respectively. It was shown that no significant difference was found in the analysis of Cortex Moutan by the developed MEKC and HPLC methods.
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References
Ding HY, Lin HC, Teng CM, Wu YC (2000) J Chin Chem Soc 47:381–388
Li G, Seo CS, Lee KS, Kim HJ, Chang HW, Jung JS, Song DK, Son JK (2004) Arch Pharm Res 27:1123–1126
Oh GS, Pae HO, Choi BM, Jeong S, Oh H, Oh C S, Rho Y D, Kim DH, Shin MK, Chung HT (2003) J Ethnopharmacol 84:85–89
Kim HK, Tak JH, Ahn YJ (2004) J Agric Food Chem 52:7857–7861
Chou TC (2003) Br J Pharmacol 139:1146–1152
Kim SA, Song GY, Kim SH (2002) Cancer Epidemiol Biomarkers Prev 11:1207S-1208S
Kim SH, Kim SA, Park MK, Kim SH, Park YD, Na HJ, Kim HM, Shin MK, Ahn KS (2004) Int Immunopharmacol 4:279–287
Yang HO, Ko WK, Kim JY, Ro HS (2004) Fitoterapia 75:45–49
Ye JF, Duan HL, Yang XM, Yan WM, Zheng XX (2001) Planta Med 67:766–767
Drasar P, Moravcova J (2004) J Chromatogr A 812:3–21
Chan TYK (1997) Drug Saf 17:209–215
Li FM, Sun SY, Wang J, Wang DW (1998) Biomed Chromatogr 12:78–85
Liang YZ, Xie PS, Chau K (2004) J Chromatogr B 812:53–70
Huang HY, Kuo KL, Hsieh YZ (1997) J Chromatogr A 771:267–274
Sheng YX, Li L, Wang C, Li YY, Guo D (2004) J Chromatogr B 806:127–132
Ye G, Li YZ, Li YY, Guo HZ, Guo D (2003) J Pharm Biomed Anal 33:521–527
Altria KD (1999) J Chromatogr A 856:443–463
Claire R L III S (1996) Anal Chem 68:569–586
Li YQ, Qi SD, Chen XG, Hu ZD (2004) Electrophoresis 25:3003–3009
Song JZ, Xu HX, Tian SJ, But PPH (1999) J Chromatogr A 857:303–311
Wang SP, Huang KJ (2004) J Chromatogr A 1032:273–279
Wu HK, Sheu SJ (1996) J Chromatogr A 753:139–146
Wang YW, Yu K, Cheng YY (2006) China J Chinese Materia Medica in press
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An erratum to this article is available at http://dx.doi.org/10.1007/s10337-011-2107-2.
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Yu, K., Wang, Y.W. & Cheng, Y. . Determination of the Active Components in Chinese Herb Cortex Moutan by MEKC and LC. Chroma 63, 359–364 (2006). https://doi.org/10.1365/s10337-006-0760-7
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DOI: https://doi.org/10.1365/s10337-006-0760-7