Abstract
Electrokinetic chromatography methods for the enantiomeric resolution of four imidazole derivatives, potential aromatase inhibitors, were developed using highly Sulfated α-, highly Sulfated β- and highly Sulfated γ- CDs as chiral selectors at acidic pH. The optimization of the various operational parameters (nature and concentration of the CD, capillary length, buffer concentration, presence of organic modifier in the electrolyte, temperature and voltage) permits to obtain resolution factors superior to 3, for each racemic analyte, with migration times of the second enantiomers less than 6 minutes. The four optimal analytical methods were validated prior to the determination of the enantiomeric purity of the eight enantiomers previously isolated and analyzed by chiral HPLC.
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Persiani S, Broutin F, Cicioni P, Stefanini P, Strolin Benedetti M (1996) Eur J Pharm Sci 4: 331–340
Auvray P, Moslemi S, Sourdaine P, Galopin S, Seralini GE, Enguehard C, Dallemagne P, Bureau R, Sonnet P, Rault S (1998) Eur J Med Chem 33:451–462
Segaloff A (1978) in: Mc Guire W L (eds.) Hormones and mammary carcinogens. Advances in Research and Treatment, Experimental Biology Plenum, New York
Njar VCO, Brodie AMH (1999) Drugs 58:233–255
Feutrie ML, Bonneterre J (1999) Bull Cancer 86:821–827
Nativelle-Serpentini C, Molesni S, Yous S, Park CH, Lesieur D, Sourdaine P, Seralini GE (2004) J Enzym Inhib Med Ch 19:119–127
Danel C (2003) Enantiomeric separation of potential aromatase inhibitors using high performance liquid chromatography and capillary electrophoresis. Thesis of the University of Lille 2
Francotte E, Junker-Buchheit A (1992) J Chromatrogr 576:1–45
Okamoto Y, Kaida Y (1994) J Chromatogr A 666:403–419
Dingenen J (1994) in: Subramanian G (eds.) A Practical Approach to Chiral Separations by Liquid Chromatography, New-York, pp. 115–181
Danel C, Foulon C, Guelzim A, Park CH, Bonte JP, Vaccher C (2005) Chirality 17:600–607
Van Eeckhaut A, Boonkerd S, Detaevernier MR, Michotte Y (2000) J Chromatogr A 903:245–254
Foulon C, Danel C, Vaccher MP, Bonte JP, Vaccher C, Goossens JF (2004) Electrophoresis 25:2735–2744
Otsuka K, Matsumura M, Kim JB, Terabe S (2003) J Pharm Biomed Anal 30:1861–1868
Chankvetadze B, Endresz G, Blaschke G (1995) J Chromatogr A 700:43–49
Wu YS, Lee HK, Li SFY (2000) Electrophoresis 21:1611–1619
Wu YS, Lee HK, Li SFY (2001) J Chromatogr A 912:171–179
Zhou L, Thompson R, Ellison D, Wyvratt JM (2004) Electrophoresis 25:2860–2865
Danel C, Lipka E, Bonte JP, Goossens JF, Vaccher C, Foulon C (2005) Electrophoresis 26:3824–3832
Chen FT, Shen G, Evangelista RA (2001) J Chromatogr A 924:523–532
Chapman J, Whatley H, Chen FT in Application information : Methods development strategy for enantiomer analysis using the P/ACE MDQ chiral system, Beckman Coulter Inc. (www.beckmancoulter.com)
Foulon C, Danel C, Vaccher C, Yous S, Bonte JP, Goossens JF (2004) J Chromatogr A 1035:131–136
Chankvetadze B (1997) Capillary Electrophoresis in Chiral Analysis, John Wiley & sons, Chichester
Issaq H, Atamna IZ, Muschik GM, Janini GM (1991) Chromatographia 32:155–161
Proceedings of the International Conference on Harmonization (ICH) of technical requirements for registration of pharmaceuticals for human use, IFPMA, Genève (1996)
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Danel, C., Foulon, C., Goossens, J.F. et al. Validation of Chiral Electrokinetic Chromatography Methods Using Highly Sulfated Cyclodextrins: Determination of Enantiomeric Purity of Aromatase Inhibitors. Chroma 63, 353–358 (2006). https://doi.org/10.1365/s10337-006-0757-2
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DOI: https://doi.org/10.1365/s10337-006-0757-2