Abstract
A new methodology was developed for analysis of aldehydes and ketones in fuel ethanol by high-performance liquid chromatography (HPLC) coupled to electrochemical detection. The electrochemical oxidation of 5-hydroxymethylfurfural, 2-furfuraldehyde, butyraldehyde, acetone and methyl ethyl ketone derivatized with 2,4-dinitrophenylhydrazine (DNPH) at glassy carbon electrode present a well defined wave at +0.94 V; +0.99 V; +1.29 V; +1.15 V and +1.18 V, respectively which are the basis for its determination on electrochemical detector. The carbonyl compounds derivatized were separated by a reverse-phase column under isocratic conditions with a mobile phase containing a binary mixture of methanol / LiClO4(aq) at a concentration of 1.0 × 10−3 mol L−1 (80:20 v/v) and a flow-rate of 1.1mL min−1 . The optimum potential for the electrochemical detection of aldehydes-DNPH and ketones-DNPH was +1.0 V vs. Ag/AgCl. The analytical curve of aldehydes-DNPH and ketones-DNPH presented linearity over the range 5.0 to 400.0 ng mL−1, with detection limits of 1.7 to 2.0 ng mL−1 and quantification limits from 5.0 to 6.2 ng mL−1, using injection volume of 20 μL. The proposed methodology was simple, low time-consuming (15 min/analysis) and presented analytical recovery higher than 95%.
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de Oliveira MF, Saczk AA, Okumura LL, Fernandes AP, Moraes M, Stradiotto NR (2004) Anal Bioanal Chem 380:135–140
de Oliveira MF, Saczk AA, Okumura LL, Stradiotto NR (2002) Ecl Quím 27:153–160
Okumura LL, Saczk AA, de Oliveira MF, Zanoni MVB, Stradiotto NR (2005) Anal Sci 21:441–444
Okumura LL, Saczk AA, de Oliveira MF, Zanoni MVB, Stradiotto NR (2005) Anal Bioanal Chem 381:1619–1624
Carbonel V, Salvador A, de La Gardia M (1992) Fresenius J Anal Chem 355:529–532.
Padilha PM, Padilha CCF, Rocha JC (1999) Anal Chem 18:299–303
Padilha PM, Gomes LA, Moreira JC, Filho NLD, Gushiken Y (1998) J Bras Chem Soc 9:494–498
Wardencki W, Orlita J, Namiesnik J. (2001) Fresenius J Anal Chem 369:661–665
Matthews TG, Howell TC (1981) J Air Pollut Control Assoc 31:1181–1184
Wells RJ (1999) J Chromatogr A 843:1–18
Rosenfeld JM (1999) J Chromatogr A 843:19–27
Vogel M, Bü A, Karst U (2000) Fresenius J Anal Chem 366:781–791
Kiss G, Varga B, Varga Puchony Z, Gelencsar A, Krivácsy Z, Hlavay J (1999) Talanta 48:755–762
García-Alonso S, Pérez-Pastor RM (1998) Anal Chim Acta 367:93–99
Stashenko EE, Ferreira MC, Sequeda LG, Martínez JR, Wong JW (1997) J Chromatogr A 779:360–369
Kissinger P T, Jacobs WA (1982) J Liq Chromatogr 5:669–676
Possanzini M, Dipalo V (1995) Chromatographia 40:134–138
Lehmpuhl DW, Birks JW (1996) J Chromatogr A 740:71–81
Shriner RC, Fugon RC, Curtin DY, Morril TC (1980) In: The Systematic Identification of Organic Compounds, (eds) John Wiley & Sons, New York
Nascimento RF, Marques JC, Lima Neto BS, Keukeleire DD, Franco DW (1997) J Chromatogr A 782:13–23
En-Chu L, Van de Mark MR (1982) J Chem Soc Chem Comm 13:1175–1178
Lee HS, Choi S J, Lee H M, Jeong CK, Kim SB, Lee JT, Yoo SD, DeLuca. PP, Lee KC (1999) Chromatographia 50:701–704
Meriluto J, Kincaid B, Smyth MR, Wasberg M (1998) J Chromatogr A 810:226-230
Fleming LH, Reynolds NC (1984) J Liq Chromatogr 7:793–8-8
Coutrim MX, Nakamura LA, Collins CH (1993) Chromatographia 37:185–190
Snyder LR, Kirkland JJ, Glajch JL (1997) In: Practical HPLC Method Development, Second edition, (eds) John Wiley & Sons, New York
Albery WJ, Beck TW, Brooks WN, Fiellenz M (1981) J Electroanal Chem 125:205–217
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Saczk, A.A., Okumura, L.L., Oliveira, M.F.d. et al. Determination of Aldehydes and Ketones in Fuel Ethanol by High-Performance Liquid Chromatography with Electrochemical Detection. Chroma 63, 45–51 (2006). https://doi.org/10.1365/s10337-005-0698-1
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DOI: https://doi.org/10.1365/s10337-005-0698-1