Abstract
We studied microemulsion electrokinetic chromatography (MEEKC) for the analysis of cefazolin, cefoperazone and cephalexin. We compared the separation of the cephalosporins obtained by MEEKC at a high pH and at a low pH. Good separations of the compounds were achieved within 9 min by both methods. To reduce the analysis time, we used the "short-end" injection technique. We also present two on-line preconcentrations of the analytes by sample stacking, reversed electrode polarity stacking mode (REPSM) (at high-pH) and stacking with reverse migrating pseudostationary phase (SRMP) (at low-pH). We also report parameters for validation such as linearity and limit of detection. Our results show that the developed methods are suitable for analyzing these three cephalosporins.
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Puig, P., Borrull, F., Calull, M. et al. Strategies for Analyzing Cephalosporins by Microemulsion Electrokinetic Chromatography. Chroma 62, 603–610 (2005). https://doi.org/10.1365/s10337-005-0685-6
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DOI: https://doi.org/10.1365/s10337-005-0685-6