Abstract
The importance of cephalosporin antibiotics in medicine requires the development of high performance separation methods for their analysis. In this paper cefazolin, cefuroxime, cefoxitin, cefaclor and ceftriaxone were used as model compounds to investigate the retention mechanism under zwitterionic HILIC conditions. The analysis was performed using a ZIC®-HILIC analytical column (150.0 × 2.1 mm i.d., particle size 3.5 μm, 200 Å) with isocratic elution. The mobile phase was a mixture of acetonitrile and ammonium acetate or formate buffer. To get a better insight into the retention mechanism on ZIC®-HILIC columns the main factors influencing the retention and selectivity of the analytes have been thoroughly investigated. It is shown that the type of the salt and its concentration along with the volume fraction of water in the mobile phase and pH of the aqueous buffer greatly affect the retention of the tested cephalosporins due to their structures and physicochemical properties. The method was applied successfully to the analysis of injection dosage forms containing cefazolin, cefuroxime and cefoxitin.
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References
Kalman D, Barriere SL (1990) Tex Heart Inst J 17:203–215
Annè S, Reisman RE (1995) Ann Allergy Asthma Immunol 74:167–170
Crotty DJ, Chen XJ, Scipione MR, Dubrovskaya Y, Louie E, Ladapo JA, Papadopoulos J (2015) J Pharm Pract 1:1–7. doi:10.1177/0897190015587254
Barriere SL (2015) Expert Opin Pharmacother 16:1–3
Kiang TK, Ensom MH (2016) Clin Pharmacokinet 55:313–358
Kiang TK, Häfeli UO, Ensom MH (2014) Clin Pharmacokinet 53:695–730
Pistos C, Tsantili-Kakoulidou A, Koupparis M (2003) J Liq Chrom Relat Tech 26:937–952
Manna L, Valvo N (2004) Chromatographia 60:645–649
Viberg A, Sandstrom M, Jansson B (2004) Rapid Commun Mass Spectrom 18:707–710
McWhinney BC, Wallis SC, Hillister T, Roberts JA, Lipman J, Ungerer JPJ (2010) J Chromatogr B 878:2039–2043
Sanli S, Sanli N, Gumustas M, Ozkan SA, Karadas N, Aboul-Enein HY (2011) Chromatographia 74:549–558
Akl MA, Ahmed MA, Ramadan A (2011) J Pharm Biomed Anal 55:247–252
Briscoe SE, McWhinney BC, Lipman J, Roberts JA, Ungerer JPJ (2012) J Chromatogr B 907:178–184
Abdelaleem EA, Naguib IA, Zaazaa HE, Hussein EA (2016) J Chromatogr Sci 54:179–186. doi:10.1093/chromsci/bmv125
Liu Q, Xu L, Kea Y, Jin Y, Zhang F, Liang X (2011) J Pharm Biomed Anal 54:623–628
Jovanović M, Rakić T, Jančić-Stojanović B, Malenović A, Ivanović D, Medenica M (2012) J Sep Sci 35:1424–1431
Hemström P, Irgum K (2006) J Sep Sci 29:1784–1821
Buszewski B, Noga S (2012) Anal Bioanal Chem 402:231–247
Santali EY, Edwards D, Sutcliffe OB, Bailes S, Euerby MR, Watson DG (2014) Chromatographia 77:873–881
Greco G, Letzel T (2013) J Chromatogr Sci 51:684–693
Guo Y (2015) Analyst 140:6452–6466
Kadar EP, Wujcik CE (2009) J Chromatogr B 877:471–476
Ruta J, Boccard J, Cabooter D, Rudaz S, Desmet G, Veuthey JL, Guillarme D (2012) J Pharm Biomed Anal 63:95–105
Georgakakou S, Kazanis M, Panderi I (2010) Anal Bioanal Chem 397:2161–2170
Vrakas D, Panderi I, Hadjipavlou-Litina D, Tsantili-Kakoulidou A (2005) Quant Struct Act Relat J 24:254–260
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Panderi, I., Malamos, Y., Machairas, G. et al. Investigation of the Retention Mechanism of Cephalosporins by Zwitterionic Hydrophilic Interaction Liquid Chromatography. Chromatographia 79, 995–1002 (2016). https://doi.org/10.1007/s10337-016-3119-8
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DOI: https://doi.org/10.1007/s10337-016-3119-8