Abstract
By plotting of the affinity of component to adsorbent (β a ) vs. the logarithm of the solvent activity in the mobile phase in a reversed phase HPLC system, it is shown that when the concentration of organic solvent in the mobile phase increases, βa of a series of aromatic alcohol homologues tends to converge to a point. A theoretical model for evaluating the convergent coordinates was presented by using the linear relationship between β a and log a D and the stoichiometric displacement theory for retention (SDT-R). The physical meaning of the coordinates of convergence point was also elucidated. Convergence occurs when the activity of the displacing solvent approaches the value when in a pure state. The experimental results fit the equations and the coordinate of the convergence point well. At the same time, a unified equation to express the quantitative relation between parameter β a describing the adsorption process in a liquid-solid system and k describing the retention process in a liquid chromatographic system is also derived.
Similar content being viewed by others
References
Lau SY, Tancja AK, Hodges RS (1984) J Chromatogr 317:129–140
Stadalius MA, Gold HS, Snyder LR (1984) J Chromatogr 296:31–59
Glajch JL, Quary MA, Vasta JF, Snyder LR (1986) Anal Chem 58:280–285
Kunitani M, Hirtzer P, Johnson D (1986) J Chromatogr 359:391–402
Quarry MA, Grob RL, Snyder LR (1984) J Chromatogr 285:1–18
Geng XD, Regnier FE (1984) J Chromatogr 296:15–30
Geng XD, Shi YL (1989) Science in China (Series B) 32:571–579
Geng XD (1994) J Norwest University (natural Sci Ed) 24:337–344
Geng XD, Bian LJ (1992) Science in China(Ser B) 35:262–271
Geng XD, Wang Y, Yu QM (2001) Acta Chimica Sinica 59:1847–1852
Geng XP, Geng XD (1993) Chinese J Chem 11:385–389
Wang Y, Geng XD (2003) Thermochimica Acta 404:109–115
Colin H, Krstulovic AM, Gonnord MF, Guiochon G (1983) Chromatographia 17:9–15
Berendsen GE, de Galan L (1980) J Chromatogr 196:21–37
Tchapla A, Colin H, Guiochon G (1984) Anal Chem56:621–625
Krstulovic AM, Colin H, Tchapla A, Guiochon G (1983) Chromatographia 17:228–230
Colin H, Guiochon G, Diez-Masa JC (1981) 53:146–155
Kaibara A, Hoha C, Hierata N (1990) Chromatograhia 292:75–288
Geng XD (1995) Science in China (Series B) 25:364–371
Geng XD (2001) Guide to theory of modern separation science. Higher Education Press of China, Beijing, pp. 262–264
Geng XD, Regnier FE (1994) Chromatographia 38:158–162
Jacobson J, Frenz J, Horvath Cs (1984) J Chromatogr 31:653–68
Huang JX, Horvath Cs (1987) J Chromatogr 406:275–284
Katsanos NA, Karaiskakis G, Agathonos P (1981) J Chromatogr 218:409–415
Adamson AW, Physical Chemistry of Surfaces, 4th ed, Wiley, New York, 1982
Stoeckli F, López-Ramón MV, Moreno-Castilla C (2001) Langmuir 11:3301–3306
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, Y., Zhang, X. & Geng, X. The Convergence Coordinates of the Affinity of a Component to the Stationary Phase vs. the Composition of Mobile Phase in HPLC. Chroma 61, 299–302 (2005). https://doi.org/10.1365/s10337-005-0504-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-005-0504-0