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The Use of 3 and 12 Micron Particulate Stationary Phases in Voltage-Assisted Micro-LC for the Separation of Mixtures Containing Neutral, Basic and Acidic Analytes

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Abstract

The application of voltage-assisted micro-LC using CEC instrumentation (capable of applying 1.2 MPa of pressure to supplement the electroosomotic flow) has been demonstrated. This has been achieved by the use of 100 μm × 24 cm capillaries packed with 12 μm stationary phase material; alternatively 7.5 cm capillaries packed with 3 μm material using the short-end injection approach with reversed polarity can be used. The paper highlights how both approaches can be successfully employed to control the separation selectivity of a range of mixtures containing acidic, basic and neutral components by the concomitant addition of voltage (either positive or negative) to the applied micro-LC flow.

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Acknowledgments.

The authors gratefully acknowledge Thermo Electron Corporation (Runcorn, UK) for kindly supplying stationary phase materials.

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Authors and Affiliations

  1. AstraZeneca R & D Charnwood/Lund, Pharmaceutical and Analytical R & D, Bakewell Rd, Loughborough, Leicestershire, LE11 5RH, England, UK

    M. R. Euerby & A. P. McKeown

  2. Department of Pharmaceutical Sciences, University of Strathclyde, The John Arbuthnott Building, 27 Taylor Street, Glasgow, G4 0NR, Scotland, UK

    B. Channer, P. U. Uhl, G. G. Skellern & D. G. Watson

Authors
  1. B. Channer
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  2. P. U. Uhl
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  3. M. R. Euerby
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  4. A. P. McKeown
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  5. G. G. Skellern
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  6. D. G. Watson
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Correspondence to M. R. Euerby.

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Channer, B., Uhl, P., Euerby, M. et al. The Use of 3 and 12 Micron Particulate Stationary Phases in Voltage-Assisted Micro-LC for the Separation of Mixtures Containing Neutral, Basic and Acidic Analytes. Chroma 61, 113–119 (2005). https://doi.org/10.1365/s10337-004-0480-9

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  • DOI: https://doi.org/10.1365/s10337-004-0480-9

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