Abstract
A method for the determination of triphenyltin and diphenyltin was developed by reversed-phase high-performance liquid chromatography with UV detection. Triphenyltin and diphenyltin were separated using a reversed-phase Symmetry C18 column (150 × 3.9 mm, 5 μm) with tetrahydrofuran-water-acetonitrile-glacial acetic acid (13:25:5:7, v/v) containing 0.05% triethylamine and 1.0% sodium acetate as mobile phase at 0.50 mL min−1 and detection at 257 nm. The calibration curves were linear from 0.26 μmol L−1 to 1100 μmol L−1 for triphenyltin with a correlation coefficient of 0.9999 (n=12) and from 0.60 μmol L−1 to 1200 μmol L−1 for diphenyltin with a correlation coefficient of 0.9991 (n=12), respectively. The detection limits of triphenyltin and diphenyltin were 0.2 μmol L−1 and 0.4 μmol L−1, respectively. The method was successfully applied to the determination of triphenyltin and its metabolite diphenyltin in culture medium. The recoveries of triphenyltin and diphenyltin were in the ranges of 97.7% to 103.3% and 85.5% to 91.6%, respectively.
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Lu, H., Li, H., Chen, F. et al. Determination of Triphenyltin and Its Metabolite Diphenyltin in Culture Medium by High-Performance Liquid Chromatography with UV detection. Chromatographia 60, 113–116 (2004). https://doi.org/10.1365/s10337-004-0328-3
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DOI: https://doi.org/10.1365/s10337-004-0328-3