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Validation of an Analytical Method for the Determination of Indolyl-3-Acryloylglycine in Human Urine Using LC-MS-MS

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Abstract

A rapid, simple and sensitive LC-MS-MS method for the determination of indolyl-3-acryloylglycine in human urine was developed and validated to internationally recognised standards. The chromatographic system used a phenyl-hexyl column with isocratic elution using acetonitrile and 0.1% formic acid. For this method, employing a stable-isotopically-labelled internal standard and non-matrix calibration standards, all coefficients of determination (r2) of the calibration lines obtained during the validation were equal to, or better than, 0.9968. Inter-batch precision ranged between 2.8% and 14.1%. Inter-batch accuracy ranged between 89.9% and 101.4%. Accordingly, the method will be suitable for use in testing the premise that elevated levels of indolyl-3-acryloylglycine in human urine are diagnostic for certain learning and behavioural disorders.

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Authors and Affiliations

  1. York Bioanalytical Solutions, Cedar House, Northminster Business Park, Northfield Lane, Upper Poppleton, York, YO26 6QF, UK

    G.A. Imrie & T.A.G. Noctor

  2. Sunderland Pharmacy School, University of Sunderland, Sunderland, SR1 3SD

    W.J. Lough

Authors
  1. G.A. Imrie
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  2. T.A.G. Noctor
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  3. W.J. Lough
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Corresponding author

Correspondence to T.A.G. Noctor.

Additional information

Presented at: 15th International Bioanalytical Forum. The Changing Role of Bioanalysis: Discovery to Market, Guildford, UK, July 1–4, 2003

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Imrie, G., Noctor, T. & Lough, W. Validation of an Analytical Method for the Determination of Indolyl-3-Acryloylglycine in Human Urine Using LC-MS-MS. Chromatographia 59 (Suppl 2), S209–S213 (2004). https://doi.org/10.1365/s10337-004-0285-x

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  • DOI: https://doi.org/10.1365/s10337-004-0285-x

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