Abstract
Efficient and novel oil-in-water microemulsion HPLC separations of a range of solutes have been achieved using isocratic elution and a conventional reversed-phase HPLC column. A single set of isocratic oil-in-water microemulsion HPLC conditions are shown to be successful for the resolution of a wide range of basic, neutral and acidic drugs and excipients. A robust separation method was developed for the quantitative analysis of Naproxen in a tablet formulation. The method offered a similar run time and improved chromatography when compared to conventional HPLC modes, demonstrating its potential for routine use. In developing the separations the effect on the chromatography of varying the operating parameters was studied. While generally the method was robust to changes in some variables, others markedly changed the separation selectivity, solute retention, peak-peak resolution or resulted in poor chromatographic performance. The method was found to be compatible with very low UV working detection wavelengths. As a final stage to the experimental programme, a microemulsion method was developed with the capability of analysing a range of water-insoluble drugs.
Similar content being viewed by others
References
Berthod A, De Carvalho M (1992) Anal Chem 64: 2267–2272
Berthod A, Laserna JJ, Carretero I (1992) JLiq Chromatogr 17: 3115–3127
Bryant S, Clark BJ, Altria K (2003) internal communication
Hernandez-Torres MA, Landy JS, Dorsey JG (1986) Anal Chem 58: 744–747
Berthod A, Nicolas O, Porthault M (1990) Anal Chem 62: 1402–1407
Khaledi MG (1997) J Chromatogr A 780: 3–40 DOI 10.1016/S0021–9673(97)00610–9
Micellar liquid chromatography, BerthodA, Garcia-Alvarez-Coque C (2000) Marcel Dekker Inc
Berthod A (1986) Adv Colloid Interface Sci 25: 3–40
Altria K, Clark BJ, Mahuzier P-E (2000) Chromatographia 52: 758–768
The Merck Index 13th Budavari S (ed) (2001) Whitehouse Station
Kulikov AU, Loginova LP, SamokhinaLV (2003) Chromatographia 57: 463
Lavine BK, Hendayana S, Tetreault J (1994) Anal Chem 66: 3458–3465
Guermouche MH, Guermouche S, HabelD (1998) Fluid Phase Equilib 147: 301–307 DOI 10.1016/S0378–3812(98)00242–8
Garcia-Alvarez-Coque MC, Torres-LapazioJR, Baeza-Baeza JJ (1997) J.Chromatogr A 780: 129–148 DOI 1016/S0021–9673(97)00051–4
Rodgers AH, Strasters JK, Khaledi MG (1993) J Chromatogr 636: 203–212 DOI 10.1016/0021–9673(93)80233-X
Kord A, Khaledi MG (1993) J Chromatogr 631:125 DOI 10.1016/0021–9673(93)80512–7
Rodriguez-Delago MA, Sanchez MJ (1994)Anal Chem Acta 298: 423 DOI 10.1016/0003–2670(94)00287–8
Lopez-Grio S, Garcia-Alvarez-Coque MC (2000) Anal Chem 72: 4826–4835 DOI 10.1021/ac000414a
Madamba-Tan L, Strasers J, Khaledi MG (1994) J Chromatogr A 683: 335–345 DOI 10.1016/0021–9673(94)00451-X
Nishi H (1997) J. ChromatogrA 780: 243–264 DOI 10.1016/S0021–9673(97)00610–9
ICH Guidelines Q2A, Text on Validation of Analytical Procedures, 1995; www.ich.org
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Marsh, A., Clark, B. & Altria, K. Oil-in-Water Microemulsion High Performance Liquid Chromatographic Analysis of Pharmaceuticals. Chromatographia 59, 531–542 (2004). https://doi.org/10.1365/s10337-004-0262-4
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1365/s10337-004-0262-4