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Determination of Dextromethorphan and Dextrorphan in Human Urine by High Performance Liquid Chromatography for Pharmacogenetic Investigations

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Abstract

The aim of the present study was to develop a sensitive method to measure dextromethorphan and dextrorphan in urine by HPLC to support pharmacogenetic studies in ethnic groups. Linearity was assessed in the range: 0.015–10 μg mL−1 for dextromethorphan and 1-10 μg mL−1 for dextrorphan. Inter and intra-day coefficients of variation were < 10%. Limits of detection and quantitation were 0.003 μg mL−1 and 0.015 μg mL−1 for dextromethorphan and 0.24 μg mL−1 and 1.0 μg mL−1 for dextrorphan, respectively. The method is reliable in helping determine the phenotype of Mexican ethnic groups using model drugs such as dextromethorphan.

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Authors and Affiliations

  1. Laboratorio de Farmacología, Instituto Nacional de Pediatría, Avenida Imán No.1, 3er piso Colonia Cuicuilco, CP 04530, México D.F., México

    J. Flores-Pérez, C. Flores-Pérez, H. Juárez-Olguín & I. Lares-Asseff

  2. Departamento de Farmacología, Facultad de Medicina, Universidad Nacional Autónoma de, México

    J. Flores-Pérez & H. Juárez-Olguín

  3. Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Durango, Instituto Politécnico Nacional, México

    I. Lares-Asseff & M. Sosa-Macías

Authors
  1. J. Flores-Pérez
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  2. C. Flores-Pérez
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  3. H. Juárez-Olguín
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  4. I. Lares-Asseff
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  5. M. Sosa-Macías
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Correspondence to J. Flores-Pérez.

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Flores-Pérez, J., Flores-Pérez, C., Juárez-Olguín, H. et al. Determination of Dextromethorphan and Dextrorphan in Human Urine by High Performance Liquid Chromatography for Pharmacogenetic Investigations. Chromatographia 59, 481–485 (2004). https://doi.org/10.1365/s10337-004-0224-x

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  • DOI: https://doi.org/10.1365/s10337-004-0224-x

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