Abstract
N-Methylpyrrolidine in cefepime for injection was determined by capillary electrophoresis with indirect UV detection. Best results were achieved with background electrolyte consisting of 10 mM creatinine adjusted to pH 3.8 with formic acid and an applied voltage of 30 kV in a bare fused-silica capillary. Indirect UV detection was performed at a wavelength of 225 nm. The application of a small amount of inlet pressure during the separation assisted the attainment of a stable baseline. The optimized method was validated regarding selectivity, linearity, accuracy, precision, ruggedness, repeatability and detection limits. Careful control of capillary conditioning enabled migration time precision values of <0.2% RSD. The use of an internal standard enabled precision values of <1% RSD to be obtained for peak area ratios.
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References
Wynd M, Paladino J (1996) Ann Pharmacother 30:1414–1424
Okamoto MP, Nakahiro RK, Chin A, Bedikian A, Gill MA (1994) Am J Hosp Pharm 51:463–477
Forgue ST, Kari P, Barbhaiya R (1987) Drug Metab Disp 15:808–815
United States Pharmacopeial Convention USP26 (2003) United States Pharmacopeial Convention, Rockville pp. 360
Fubara JO, Notari RE (1998) J Pharm Sci 87:1572–1576
Divjak B, Goessler W, Haddad PR, Novic M (2003) J Chromtogr A 1008:89–96
Zhou H (2003) Sepu 21:184–186
Beckers JL, Bocek P (2003) Electrophoresis 24:518–535
Pospichal J, Gebauer P, Bocek P (1989) Chem Rev 89:419–430
Andersson EKM, Hagglund I (2002) J Chromtogr. A 979:11–25
Kenndler E (1998) In High Performance Capillary Electrophoresis: Khaledi MG (ed) John Wiley & Sons, New York pp. 35
Andersson EKM, Hagglund I (2002) J Chromtogr A 953:227–237
Altria KD (2002) LCGC Eur September, 1–5
Krivankova L, Gebauer P, Pantuckova P, Bocek P (2002) Electrophoresis 23:1833–1843
Mayer BX (2001) J Chromtogr A 907:21–37
Evagelou V, Tsantili-Kakoulidou A, Koupparis M. (2003) J Pharm Biomed Anal 31:1119–1128
Johns C, Shaw MJ, Macke M, Haddad PR (2003) Electrophoresis 24:557–566
Johns C, Macke M, Haddad PR (2003) J Chromtogr A 997:87–94
Schoftner R, Pfeifer A, Buchberger W (2002) J Sep Sci 25:507–513
Altria KD, Wood T, Kitscha R, Roberts-McItosh A (1995) J Pharm Biomed Anal 13:33–38
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Liu, H., Sunderland, V. Determination of N-Methylpyrrolidine in Cefepime for Injection by Capillary Electrophoresis with Indirect UV Detection. Chromatographia 59, 653–657 (2004). https://doi.org/10.1365/s10337-004-0191-2
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DOI: https://doi.org/10.1365/s10337-004-0191-2