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Simultaneous Determination of Cefixime, Cefdinir and Clavulanic Acid by High Performance Liquid Chromatography

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Pharmaceutical Chemistry Journal Aims and scope

In this study, two cephalosporin groups of antibiotics (cefepime and cefixime) and their combinations with clavulanic acid, beta lactamase inhibitor, were simultaneously analyzed by HPLC method. The optimum separation was carried out on MZ C18 column (250 × 4.6 mm; i.d. 5 μm) with mobile phase consisting of an acetonitrile – 0.01% phosphoric acid (pH 2.5) (15 : 85, v/v) binary mixture. Detection was performed using a diode array detector at 228 nm. Under these experimental conditions, the analysis was accomplished in about 10 min. The developed method is specific, linear, sensitive, accurate, precise, and robust. The method was completely validated showing satisfactory data for all the parameters tested. Finally, the proposed method was successfully applied to two combined pharmaceutical dosage forms containing cefepime with clavulanic acid and cefixime with clavulanic acid.

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References

  1. H. S. Sader,M. R. Jacobs, and T. R. Fritsche, Diagn. Microbiol. Infect. Dis., 57(3), 5 – 12 (2007).

    Article  Google Scholar 

  2. P. S. Saudagar, S. A. Survase, and R. S. Singhal, Biotechnol. Adv., 26(4), 335 – 351 (2008).

    Article  CAS  Google Scholar 

  3. P. B. Shah and K. Pundarikakshudu, J. AOAC Int., 89(4), 987 – 994 (2006).

    Article  CAS  Google Scholar 

  4. Z. Talebpour, H. Pourabdollahi, H. Rafati, et. al., Sci. Pharm., 81(2), 493 – 504 (2013).

    Article  CAS  Google Scholar 

  5. H. Hashem, A. A. Gouda, and W. Hassan, J. Liq. Chromatogr. Relat. Technol., 35(12), 1638 – 1648 (2012).

    Article  CAS  Google Scholar 

  6. M. Kumudhavalli, R. M. Chandira, B. Jayakar, et. al., J. Pharm. Res., 2(6) (2009).

  7. W. Xing-lin, Chin. New Drugs J., 2, 010 (2003).

    Google Scholar 

  8. S. D. Bhinge and S. M. Malipatil, J. Taibah Univ. Sci., 10(5), 734 – 744 (2016).

    Google Scholar 

  9. P. Deekonda and M. S. Reddy, Der Pharm. Chem., 6(2), 31 – 37 (2014).

    Google Scholar 

  10. G. Devika, M. Sudhakar, and J. V. Rao, Orient J. Chem, 28(4), 1743 – 1750 (2012).

    Article  CAS  Google Scholar 

  11. M. V. Dhoka, S. J. Sandage, and S. C. Dumbre, J. AOAC Int., 93(2), 531 – 535 (2010).

    CAS  PubMed  Google Scholar 

  12. G. Rathinavel, J. Valarmathy, L. Samueljoshua, et. al., E-J. Chem., 5(3), 648 – 651 (2008).

  13. S. Kathiravan and S. Mathankumar, Asian J. Chem, 3(4), 865 – 868 (2010).

    Google Scholar 

  14. G. J. Kher, V. R. Ram, G. G. Pandiya, et. al., Int. J. Chemtech. Res., 4(3), 1124 – 1136 (2012).

    CAS  Google Scholar 

  15. M. M. Mahesh, V. S. Kasture, and S. A. Gosavi, Eurasian J. Anal. Chem., 5(3), 227 – 238 (2010).

    Google Scholar 

  16. S. Natesan, Int. J. Pharm. Sci. Rev. Res, 2(2), 219 – 224 (2016).

    Google Scholar 

  17. N. Nyola and S. Jeyabalan, Indo Am. J. Pharm. Res., 2(12), 1472 – 1481 (2013).

    Google Scholar 

  18. N. S. Patel, F. B. Tandel, Y. D. Patel, et. al., Indian J. Pharm. Sci., 76(6), 535 (2014).

    Google Scholar 

  19. S. A. Patel and J. V. Patel, Int. J. Pharm., Chem. Biol. Sci., 3(2), 372 – 379 (2013).

  20. S. A. Patel and J. V. Patel, Int. J. Pharm. Tech. Res., 3(4), 1958 – 1962 (2011).

    CAS  Google Scholar 

  21. K. J. Trivedi, P. V. Chokshi, and N. S. Patel, Int. J. Chem. Tech. Res., 4(4), 1628 – 1632 (2012).

    CAS  Google Scholar 

  22. A. R. Wankhede, Int. J. Pharm. Bio. Arch., 1(3) (2010).

  23. S. R. Narala and K. Saraswathi, J. Pharm. Sci. Res., 3(1), 1002 – 1004 (2011).

    CAS  Google Scholar 

  24. Z. G. K. X. R. Ping and G. S. C. Hui, China Pharm., 9, 012 (2008).

  25. M. A. Abounassif, E. M. Abdel-Moety,M. E. Mohamed, et. al., J. Pharm. Biomed. Anal., 9(9), 731 – 735 (1991).

    Article  CAS  Google Scholar 

  26. A. Aghazadeh and G. Kazemifard, J. Sci. I. R. Iran, 12(2), 127 – 131 (2001).

    CAS  Google Scholar 

  27. A. Aghazadeh and G. Kazemifard, J. Pharm Bio. Anal., 25(2), 325 – 329 (2001).

    CAS  Google Scholar 

  28. S. B. Dighe, Int. J. Pharm. Sci. Res., 5(4), 1566 (2014).

    Google Scholar 

  29. F. Hasanpour, A. A. Ensafi, and T. Khayamian, Anal. Chim. Acta, 670(1 – 2), 44 – 50 (2010).

  30. P. L. Ingale, S. D. Dalvi, D. D. Jadav, et. al., J. Pharm. Pharm. Sci., 5(4), 179 – 181, (2013).

    CAS  Google Scholar 

  31. S. Jadhav, V. Salunkhe, and S. Bhinge, Curr. Pharm. Res., 3(4), 994 (2013).

    Article  CAS  Google Scholar 

  32. S. Malathi, R. Dubey, and R. Venkatnarayanan, Indian J. Pharm. Sci., 71(1), 102 (2009).

    Article  CAS  Google Scholar 

  33. M. R. Sengar, S. V. Gandhi, U. P. Patil, et. al., Int. J. Chem. Tech. Res, 1(4), 1105 – 1108 (2009).

  34. G. Yu-long, Hebei Chem. Eng. Ind., 5, 034 (2008).

    Google Scholar 

  35. J. Zhou, T. Shen, and X. Lu. Chinese Pharm. J., 39(7), 538 – 539 (2004).

  36. A. Basu, K. Basak, M. Chakraborty, et. al., J. Pharm Res., 4(5), 1319 – 1321 (2011).

  37. M. P. Joshi, K. G. Gamit, V. K. Parmar, et. al., World J. Pharm. Sci., 3(9), 1259 – 1271 (2014).

  38. I. U. Khan, S. Sharif, M. Ashfaq, et. al., J. AOAC Int., 91(4), 744 – 749 (2008).

    Article  CAS  Google Scholar 

  39. S. Zade, V. Bhatpalliwar, N. Mendhule, et. al., Int. J. Pharm. Sci. Nanotech., 6(2), 2033 – 2039 (2013).

  40. ICH Guidelines. Validation of Analytical Procedures: Text and Methodology Q2 (R1), International Conference on Harmonization, Geneva (2005), pp. 11 – 12.

    Google Scholar 

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Authors and Affiliations

  1. Department of Analytical Chemistry Faculty of Pharmacy, Hacettepe University, 06100 Sýhhiye, Ankara, Türkiye

    Tuba Reçber, Ece Özkan, Emirhan Nemutlu & Sedef Kır

Authors
  1. Tuba Reçber
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  2. Ece Özkan
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  3. Emirhan Nemutlu
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  4. Sedef Kır
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Correspondence to Emirhan Nemutlu.

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Reçber, T., Özkan, E., Nemutlu, E. et al. Simultaneous Determination of Cefixime, Cefdinir and Clavulanic Acid by High Performance Liquid Chromatography. Pharm Chem J 54, 1186–1191 (2021). https://doi.org/10.1007/s11094-021-02341-z

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  • DOI: https://doi.org/10.1007/s11094-021-02341-z

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