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Degree of Complexation of Anticoagulant Rodenticides with Human Serum Albumin: Effect of Saccharose and Sodium

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Abstract

A theoretical treatment has been developed to describe association between rodenticides and human serum albumin (HSA). The degree of complexation n c (the amount (%) of complexed guest) and the association constant (K) for rodenticide–HSA binding were determined. Enthalpy–entropy compensation was also investigated in relation to this mathematical model to confirm rodenticide binding behavior with HSA. The role of the sodium cation and saccharose in this association were also analyzed. As expected for a salting-out agent, addition of Na+ cation to the mobile phase led to enhancement of n c and K values by increasing the hydrophobic effect. When saccharose was increased above a critical x c value equal to 4 mM, a decrease of the association constant and the degree of complexation was observed, owing to reduction of the radius of curvature of the HSA cavity.

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Authors and Affiliations

  1. Equipe des Sciences Séparatives et Biopharmaceutiques (2SB), Laboratoire de Chimie Analytique, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, 25030, Besançon cedex, France

    C. André & Y.C. Guillaume

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  1. C. André
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  2. Y.C. Guillaume
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Correspondence to Y.C. Guillaume.

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André, C., Guillaume, Y. Degree of Complexation of Anticoagulant Rodenticides with Human Serum Albumin: Effect of Saccharose and Sodium. Chromatographia 59, 429–436 (2004). https://doi.org/10.1365/s10337-004-0189-9

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  • DOI: https://doi.org/10.1365/s10337-004-0189-9

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