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Analysis of Drug–Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin

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Affinity Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1286))

Abstract

High-performance affinity chromatography (HPAC) is a type of liquid chromatography that has seen growing use as a tool for the study of drug–protein interactions. This report describes how HPAC can be used to provide information on the number of binding sites, equilibrium constants, and changes in binding that can occur during drug–protein interactions. This approach will be illustrated through recent data that have been obtained by HPAC for the binding of sulfonylurea drugs and other solutes to the protein human serum albumin (HSA), and especially to forms of this protein that have been modified by non-enzymatic glycation. The theory and use of both frontal analysis and zonal elution competition studies in such work will be discussed. Various practical aspects of these experiments will be presented, as well as factors to consider in the extension of these methods to other drugs and proteins or additional types of biological interactions.

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Acknowledgements

This work was supported, in part, by the National Institutes of Health under grants R01 DK069629 and R01 GM044931. Additional support for R. Matsuda was obtained through a fellowship from the Molecular Mechanisms of Disease Program at the University of Nebraska-Lincoln.

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Authors and Affiliations

  1. Department of Chemistry, University of Nebraska-Lincoln, 704 Hamilton Hall, 639 N 12 Street, Lincoln, NE, 68588-0304, USA

    Ryan Matsuda, Jeanethe Anguizola, Krina S. Hoy & David S. Hage

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  1. Ryan Matsuda
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  2. Jeanethe Anguizola
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  3. Krina S. Hoy
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  4. David S. Hage
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Correspondence to David S. Hage .

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  1. Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany

    Senta Reichelt

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Matsuda, R., Anguizola, J., Hoy, K.S., Hage, D.S. (2015). Analysis of Drug–Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin. In: Reichelt, S. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 1286. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2447-9_21

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  • DOI: https://doi.org/10.1007/978-1-4939-2447-9_21

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2446-2

  • Online ISBN: 978-1-4939-2447-9

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