Skip to main content
Log in

Pulse radiolysis study on the free radical scavenging activities of puerarin

  • Published:
Science in China Series B: Chemistry Aims and scope Submit manuscript

Abstract

The free radical scavenging activities of puerarin towards nitrogen dioxide radicals (NO ˙2 ), nitric oxide radicals (NO˙) and hydroxyl radicals (OH˙) were investigated by pulse radiolysis. Puerarin reacted with (NO ˙2 ) or (NO˙) to form puerarin-4-O˙ with the absorption maximum at 340 nm, but reacted with OH˙ to form at least three transient species including puerarin-2-O˙, puerarin-4-O˙ and [puerarin-OH]˙with a broad absorption spectrum band (300-750 nm). In addition, the reaction rate constants of puerarin reacting with NO ˙2 , and NO˙ were determined as 2.6 × 108, 1.7× 108 and 3.9 × 109 L · mol−1 · s−1, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Michael, J. T., The role of free radicals and antioxidants, Nutrition, 2000, 16(7): 716–718.

    Article  Google Scholar 

  2. Hollinan, P. C. H., Katan, M. B., Dietary flavonoids: intake, health effects and bioavailability, Food and Chemical Toxicology, 1999, 37(9): 937–942.

    Article  Google Scholar 

  3. Wang Fuwen, Wang Leiyi, Xu Shulan et al., Effect of puerarin on cerebral circulation and cerebral metabolism in rabbit, Shi Zhen Medicine and Materia Medica Research (in Chinese), 2000, 11 (7): 590–591.

    Google Scholar 

  4. Liu Qi-gong, Wang Lin, Lu Zai-ying et al., The protective effect of puerarin on ischémie myocardium in dogs and its possible mechanism, J. Clin. Cardial. (in Chinese), 1998, 14 (5): 292–295.

    Google Scholar 

  5. Duan Shu, Li Yi-fu, Luo Xiao-Ian, Effect of puerarin on heart function and serum oxidaized-LDL in the patients with chronic cardiac failure, Bull. Hunan Med. Univ. (in Chinese), 2000, 25 (2): 176–177.

    Google Scholar 

  6. Lu, X. R., Zhu, Q. L., Effects of puerarin on experimental hyperlipidemia in rabbits, Naunyn-Schmiedebergs Archives of Pharmacology (in Chinese), 1998, 358(1): 519–520.

    Google Scholar 

  7. Guerra, M. C., Speroni, E., Broccoli, M., Comparison between Chinese medical herb pueraria lobata crude extract and its main isoflavone puerarin antioxidant properties and effects on rat liver CYP-catalysed drug metabolism, Life Sciences, 2000, 67 (24): 2997–3006.

    Article  CAS  Google Scholar 

  8. Mahal, H. S., Sharma, H. S., Mukherjee, T., Antioxidant properties of melatonin: A pulse radiolysis study, Free Radical Biology & Medicine, 1999, 26 (5): 557–565.

    Article  CAS  Google Scholar 

  9. Grätzel, M., Henglein, A., Lilie, J. et al., Pulse radiolytic study of some elementary processes of nitrite ion oxidation and reduction, Ber. BunsengesPhys. Chem., 1969, 73 (2): 646–653.

    Google Scholar 

  10. Eric, J. L., Ren, S., Huynh, H. B., Quantitatie structure-activity relationship analysis of phenolic antioxidants, Free Radical Biology & Medicine, 1999, 26 (5): 285–294.

    Google Scholar 

  11. György, I., Antus, S., Blazovics, A. et al., Substituent effects in the free radical reactions of silybin: radiation-induced oxidation of the flavonoid atneutral pH, Int. J. Radiat. Biol., 1992, 61 (5): 603–609.

    Article  Google Scholar 

  12. György, I., Antus, S., Blazovics, A. et al., Pulse radiolysis of silybin: One-electron oxidation of the flavonoid atnetrual pH, Radiat. Phys. Chem., 1992, 39(1): 81–84.

    Google Scholar 

  13. Zeev, B. A., Robert, H. S., Reaction of azide radicals with aromatic compounds, Azide as a selective oxidant, J. Phys. Chem., 1985, 89(15): 3359–3363.

    Article  Google Scholar 

  14. Julia, P., Johanna, D., Flavonoids: Dietary occurrence and biochemical activity, Nutrition Research, 1998, 18 (12): 1995–2018.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Zhao Yaping.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yaping, Z., Wenfeng, W., Wenli, Y. et al. Pulse radiolysis study on the free radical scavenging activities of puerarin. Sc. China Ser. B-Chem. 46, 132–136 (2003). https://doi.org/10.1360/03yb9019

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1360/03yb9019

Keywords

Navigation